State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, P. R. China.
Adv Healthc Mater. 2021 Mar;10(6):e2001369. doi: 10.1002/adhm.202001369. Epub 2021 Jan 14.
Owing to the insufficient guidance of new bone formation in orthopedic and craniomaxillofacial surgery, construction of a guided bone regeneration membrane to support vascularized bone regeneration remains a challenge. Herein, an electrospun asymmetric double-layer polycaprolactone/collagen (PCL/Col) membrane modified by metal-organic framework (MOF) crystals is developed. The optimization of the PCL/Col weight ratio (1:1 and 1:1.5) enables the composite membrane with a balanced tensile strength (only fell by 49.9% in wet conditions) and a controlled degradation rate (completely degraded at 12 weeks). The MOF crystals can provide a pH-responsive release of Zn ions. In vitro experiments indicate that the barrier layer functions to prevent the infiltration of fibrous connective tissue. The MOF crystal layer functions to enhance osteogenesis and angiogenesis in vitro. Using a rat calvarial defect model, the MOF crystals exhibit a sign of osteoinductivity along with blood vessel formation after 8 weeks post-surgery. Strikingly, when assessed in a chick chorioallantoic membrane model, the MOF modified membrane demonstrates a significant angiogenic response, which can be envisaged as its outstanding merits over the commercially Col membrane. Therefore, the MOF crystals represent an exciting biomaterial option, with neovascularization capacity for bone tissue engineering and regenerative medicine.
由于在骨科和颅面外科中新生骨形成的指导不足，构建支持血管化骨再生的引导骨再生膜仍然是一个挑战。在此，开发了一种通过金属有机骨架（MOF）晶体修饰的静电纺丝不对称双层聚己内酯/胶原蛋白（PCL/Col）膜。PCL/Col 重量比（1:1 和 1:1.5）的优化使复合膜具有平衡的拉伸强度（仅在潮湿条件下下降 49.9%）和可控的降解率（12 周完全降解）。MOF 晶体可以提供 pH 响应性的 Zn 离子释放。体外实验表明，屏障层的功能是防止纤维结缔组织的渗透。MOF 晶体层在体外具有增强成骨和血管生成的作用。使用大鼠颅骨缺损模型，术后 8 周，MOF 晶体显示出成骨和血管形成的迹象。引人注目的是，在用鸡胚绒毛尿囊膜模型评估时，MOF 修饰的膜表现出显著的血管生成反应，这可以被视为其优于商业 Col 膜的突出优点。因此，MOF 晶体代表了一种令人兴奋的生物材料选择，具有用于骨组织工程和再生医学的新生血管形成能力。
