Wang Tianqi, Xie Qi, Liang Hongbo, Sun Yu, Xie Weili
The First Affiliated Hospital of Harbin Medical University, School of Stomatology, Harbin Medical University, Harbin, China.
School of Materials Science and Engineering, Harbin Institute of Technology, Harbin, China.
Front Bioeng Biotechnol. 2025 Jun 27;13:1611948. doi: 10.3389/fbioe.2025.1611948. eCollection 2025.
Guided bone regeneration (GBR) is widely used for maxillofacial defects, but fabricating membranes that enhance osteoinduction and antimicrobial resistance remains challenging. This study addresses critical bone defect therapy by developing collagen (Col) and zeolitic imidazolate framework-8 (ZIF-8) reinforced poly(lactide-co-glycolide) (PLGA) nanofibrous membranes.
Material characterization analyzed the composite nanofibrous membranes' morphology, structure, wettability, tensile strength, degradation, and ion release. Biocompatibility and osteogenesis were evaluated using live/dead staining, cytoskeleton staining, CCK-8 assay, alkaline phosphatase (ALP) activity quantification, ALP staining, alizarin red S (ARS) staining and ARS quantification. Antibacterial efficacy was assessed via agar plate counting and bacterial growth kinetics. bone regeneration was examined in a rat cranial critical defect model treated with the membrane; bone formation was evaluated by Micro-CT and hematoxylin-eosin staining after 4 weeks.
Optimization of the PLGA/Col weight ratio (100:3) yielded composite membranes demonstrating superior tensile strength. The PLGA/Col/ZIF-8 nanofibrous composite incorporating 1 wt% ZIF-8 nanoparticles exhibited optimal biocompatibility with sustained Zn release kinetics. experiments demonstrated that sustained release of Zn has the dual effects of stimulating osteogenic differentiation and effectively preventing early bacterial infection. a rat calvarial defect model further confirmed the positive bone regeneration effect of the PLGA/Col/ZIF-8 composite nanofibrous membrane.
PLGA/Col/ZIF-8 composite nanofibrous membranes have great potential for application in guiding bone tissue regeneration.
引导骨再生（GBR）广泛应用于颌面缺损修复，但制备具有增强骨诱导和抗菌性能的膜仍然具有挑战性。本研究通过开发胶原蛋白（Col）和沸石咪唑酯骨架-8（ZIF-8）增强的聚（丙交酯-共-乙交酯）（PLGA）纳米纤维膜来解决严重骨缺损治疗问题。
材料表征分析了复合纳米纤维膜的形态、结构、润湿性、拉伸强度、降解和离子释放。使用活/死染色、细胞骨架染色、CCK-8 测定、碱性磷酸酶（ALP）活性定量、ALP 染色、茜素红 S（ARS）染色和 ARS 定量评估生物相容性和成骨能力。通过琼脂平板计数和细菌生长动力学评估抗菌效果。在用该膜治疗的大鼠颅骨临界缺损模型中检查骨再生情况；4周后通过显微CT和苏木精-伊红染色评估骨形成。
PLGA/Col重量比（100:3）的优化产生了具有优异拉伸强度的复合膜。含有1 wt% ZIF-8纳米颗粒的PLGA/Col/ZIF-8纳米纤维复合材料表现出最佳的生物相容性和持续的锌释放动力学。实验表明，锌的持续释放具有刺激成骨分化和有效预防早期细菌感染的双重作用。大鼠颅骨缺损模型进一步证实了PLGA/Col/ZIF-8复合纳米纤维膜的积极骨再生效果。
PLGA/Col/ZIF-8复合纳米纤维膜在引导骨组织再生方面具有巨大的应用潜力。
