Department of Food Science and Technology, The Ohio State University, Columbus, OH, USA.
J Appl Microbiol. 2021 Aug;131(2):844-854. doi: 10.1111/jam.15005. Epub 2021 Feb 9.
To develop a broadly applicable medium free of proteins with well-defined and reproducible chemical composition for the cultivation of various micro-organisms with food safety significance.
The defined medium was designed as a buffered minimal salt medium supplemented with amino acids, vitamins, trace metals and other nutrients. Various strains commonly used for food safety research were selected to test the new defined medium. We investigated single growth factors needed by different strains and the growth performance of each strain cultivated in the defined medium. Results showed that the tested strains initially grew slower in the defined medium compared to tryptic soy broth, but after an overnight incubation cultures from the defined medium reached adequately high cell densities.
The newly designed defined medium can be widely applied in food safety studies that require media with well-defined chemical constituents.
Defined media are important in studies of microbial metabolites and physiological properties. A defined medium capable of cultivating different strains simultaneously is needed in the food safety area. The new defined medium has broader applications in comparing different strains directly and provides more reproducible results.
开发一种不含蛋白质、具有明确且可重复的化学成分的广泛适用的培养基，用于培养具有食品安全意义的各种微生物。
该定义培养基被设计为一种缓冲最小盐培养基，补充了氨基酸、维生素、痕量金属和其他营养素。选择了常用于食品安全研究的各种菌株来测试新的定义培养基。我们研究了不同菌株所需的单一生长因子以及在定义培养基中培养的每个菌株的生长性能。结果表明，与胰蛋白酶大豆肉汤相比，测试菌株在定义培养基中的初始生长速度较慢，但经过一夜孵育，定义培养基中的培养物达到了足够高的细胞密度。
新设计的定义培养基可广泛应用于需要化学成分明确的培养基的食品安全研究。
定义培养基在微生物代谢物和生理特性的研究中非常重要。食品安全领域需要一种能够同时培养不同菌株的定义培养基。新的定义培养基在直接比较不同菌株方面具有更广泛的应用，并提供更可重复的结果。
