Gao Le, Wu Zhitao, Ibrahim Abdul-Rauf, Zhou Shu-Feng, Zhan Guowu
College of Chemical Engineering, Integrated Nanocatalysts Institute (INCI), Huaqiao University, 668 Jimei Blvd., Xiamen, Fujian 361021, P. R. China.
Department of Mechanical Engineering, Faculty of Engineering and Built Environment, Tamale Technical University, Education Ridge Avenue, Sagnarigu District, Tamale, Ghana.
ACS Biomater Sci Eng. 2020 Nov 9;6(11):6095-6107. doi: 10.1021/acsbiomaterials.0c01152. Epub 2020 Oct 16.
Hollow nanomaterials have been used as an attractive platform for the integration of multiple bioactive components for effective anticancer therapy. Herein, we report a novel and facile strategy for the fabrication of hollow and monodispersed zeolitic imidazolate framework-8 (ZIF-8) by the self-template method with folic acid (FA) as a bioetcher. Gold nanocluster and folic acid were critical for the formation of the hollow ZIF-8 (thickness of 38 nm) during solvothermal synthesis. By integrating CuS nanoparticles (size of 4.9 nm), the resultant quadruple ZIF-8/Au/CuS/FA nanocomposites (denoted as FACZ) exhibited effective anticancer activities on FA receptor-positive MCF-7 and HepG-2 tumor cells but a weak killing effect on HCMEC/D3 cells. Folic acid molecules were conjugated to the external surface of FACZ, which simultaneously offered an excellent tumor-targeting ability and fluorescence imaging property. Although the photothermal therapy caused by CuS was not so obvious due to partial reduction, the nanosized FACZ after cellular uptake was able to release Cu(I) to enable chemodynamic therapy. This catalytically decomposed HO to produce highly reactive oxygen species via the Fenton-like reaction as determined by the extracellular and intracellular hydroxyl radical. Our work offers a simple route for the fabrication of hollow ZIF-8 nanocomposite with active and selective anticancer activity. This is envisaged to have great potentials in biomedical applications.
中空纳米材料已成为整合多种生物活性成分以实现有效抗癌治疗的有吸引力的平台。在此，我们报告了一种新颖且简便的策略，通过以叶酸（FA）作为生物蚀刻剂的自模板法制备中空且单分散的沸石咪唑酯骨架-8（ZIF-8）。金纳米簇和叶酸在溶剂热合成过程中对中空ZIF-8（厚度为38 nm）的形成至关重要。通过整合硫化铜纳米颗粒（尺寸为4.9 nm），所得的四重ZIF-8/Au/CuS/FA纳米复合材料（记为FACZ）对FA受体阳性的MCF-7和HepG-2肿瘤细胞表现出有效的抗癌活性，但对HCMEC/D3细胞的杀伤作用较弱。叶酸分子共轭到FACZ的外表面，同时提供了出色的肿瘤靶向能力和荧光成像特性。尽管由于部分还原，硫化铜引起的光热疗法不太明显，但细胞摄取后的纳米级FACZ能够释放Cu(I)以实现化学动力疗法。通过细胞外和细胞内羟基自由基测定，这通过类芬顿反应催化分解HO以产生高活性氧物种。我们的工作为制备具有活性和选择性抗癌活性的中空ZIF-8纳米复合材料提供了一条简单途径。预计这在生物医学应用中具有巨大潜力。
