Im Pilseon, Kim Jaeyun
ACS Biomater Sci Eng. 2018 Oct 8;4(10):3498-3505. doi: 10.1021/acsbiomaterials.8b00911. Epub 2018 Sep 24.
The macroscale delivery system has been one of the practical platforms for a controlled delivery system by acting as a local depot close to the target tissue. In this study, we fabricated a macroporous alginate crygel incorporated with gold nanorods (GNRs) for the on-demand release of a chemotherapeutic drug from macroscale materials placed beside the target tumor. The macroporous crygel was prepared by the ice-crystal templating of a covalently crosslinked alginate hydrogel incorporated with GNRs. Mitoxantrone (MX), one of the potent anticancer drugs with a positive charge, was strongly adsorbed on the negative alginate chains of the cryogels. This system enabled a high loading of MX and a successful on-demand release of strongly bound MX from the GNR-loaded macroporous cryogels by near-infrared (NIR) irradiation by the dissociation of the interaction between the alginate backbone and MX. Cell viability after the NIR irradiation of the MX-loaded macroporous cryogel was significantly lower compared to that under no stimuli conditions. The in vivo test showed that repetitive NIR irradiations on the MX-loaded cryogel implanted near the tumor suppressed the tumor volume six times more than that of the control group. This simple approach to fabricate a macroporous cryogel capable of the on-demand release of bioactive cargos could be beneficial in various applications including cell, gene, and the other small molecule delivery systems.
宏观递送系统通过作为靠近靶组织的局部储存库，一直是可控递送系统的实用平台之一。在本研究中，我们制备了一种掺入金纳米棒（GNRs）的大孔海藻酸盐冷冻凝胶，用于从置于靶肿瘤旁边的宏观材料中按需释放化疗药物。大孔冷冻凝胶是通过对掺入GNRs的共价交联海藻酸盐水凝胶进行冰晶模板化制备的。米托蒽醌（MX）是一种带正电荷的强效抗癌药物，它强烈吸附在冷冻凝胶的负电荷海藻酸链上。该系统能够实现MX的高负载，并通过近红外（NIR）照射使负载GNRs的大孔冷冻凝胶中强结合的MX通过海藻酸主链与MX之间相互作用的解离而成功按需释放。与无刺激条件下相比，负载MX的大孔冷冻凝胶经近红外照射后的细胞活力显著降低。体内试验表明，对植入肿瘤附近的负载MX的冷冻凝胶进行重复近红外照射，其肿瘤体积的抑制程度比对照组高六倍。这种制备能够按需释放生物活性物质的大孔冷冻凝胶的简单方法，在包括细胞、基因和其他小分子递送系统在内的各种应用中可能是有益的。
