Zhao Shuang, Xie Kai, Guo Yu, Tan Jia, Wu Junxiang, Yang Yangzi, Fu Penghuai, Wang Lei, Jiang Wenbo, Hao Yongqiang
Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
ACS Biomater Sci Eng. 2020 Sep 14;6(9):5120-5131. doi: 10.1021/acsbiomaterials.9b01911. Epub 2020 Aug 3.
Polycaprolactone (PCL) is widely used in bone tissue engineering due to its biocompatibility and mechanical strength. However, PCL is not biologically active and shows poor hydrophilicity, making it difficult for new bones to bind tightly to its surface. Magnesium (Mg), an important component of natural bone, exhibits good osteo-inductivity and biological activity. Therefore, porous PCL/Mg scaffolds, including pure PCL, PCL/5%Mg, PCL/10%Mg, and PCL/15%Mg, were prepared to elucidate whether the porous structure of scaffolds and the bioactivity of PCL may be enhanced via 3D printing and incorporation of Mg powder. Compared with the control group (pure PCL only), the hydrophilicity of composite PCL/Mg scaffolds was greatly increased, resulting in the scaffolds having decreased water contact angles. Tests for adhesion and proliferation of rat bone marrow mesenchymal stem cells (rBMSCs) indicated that the PCL/10%Mg scaffold showed superior compatibility. Furthermore, as indicated by alkaline phosphatase (ALP) activity and semiquantitative analysis of alizarin red staining, PCL/10%Mg scaffolds exhibited significantly stronger osteogenic activity than the other scaffolds. Animal experiments demonstrated that PCL/10%Mg scaffolds displayed pro-osteogenic effects at an early stage (4 weeks) and produced more new bone mass 8-12 weeks following implantation, compared with the control group. Visceral and blood parameter analyses indicated that PCL/10%Mg scaffolds did not exert any noticeable toxic effects. PCL/10%Mg composite scaffolds were found to promote bone defect repair at an early stage with good cytocompatibility. This finding revealed a new concept in designing bone tissue materials, which showed potential as a clinical treatment for bone defects.
聚己内酯（PCL）因其生物相容性和机械强度而被广泛应用于骨组织工程。然而，PCL没有生物活性，亲水性较差，这使得新骨难以紧密结合在其表面。镁（Mg）是天然骨的重要组成部分，具有良好的骨诱导性和生物活性。因此，制备了包括纯PCL、PCL/5%Mg、PCL/10%Mg和PCL/15%Mg在内的多孔PCL/Mg支架，以阐明支架的多孔结构和PCL的生物活性是否可以通过3D打印和掺入镁粉来增强。与对照组（仅纯PCL）相比，复合PCL/Mg支架的亲水性大大提高，导致支架的水接触角减小。大鼠骨髓间充质干细胞（rBMSC）的粘附和增殖测试表明，PCL/10%Mg支架表现出优异的相容性。此外，碱性磷酸酶（ALP）活性和茜素红染色的半定量分析表明，PCL/10%Mg支架表现出比其他支架更强的成骨活性。动物实验表明，与对照组相比，PCL/10%Mg支架在早期（4周）显示出促骨生成作用，并且在植入后8-12周产生更多的新骨量。内脏和血液参数分析表明，PCL/10%Mg支架没有产生任何明显的毒性作用。发现PCL/10%Mg复合支架在早期促进骨缺损修复，具有良好的细胞相容性。这一发现揭示了骨组织材料设计的一个新概念，显示出作为骨缺损临床治疗方法的潜力。
