Demehri Sharlene, Vardar Saynur, Godoy Cristina, Lopez Jose V, Samuel Paisley, Kawai Toshihisa, Ozga Andrew T
Department of Periodontology, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL 33328, USA.
Department of Oral Science and Translational Research, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL 33328, USA.
Microorganisms. 2024 Sep 21;12(9):1921. doi: 10.3390/microorganisms12091921.
Trillions of microbes comprise the human oral cavity, collectively acting as another bodily organ. Although research is several decades into the field, there is no consensus on how oral microbiomes differ in underrepresented groups such as Hispanic, Black, and Asian populations living in the United States. Here, using 16S ribosomal RNA sequencing, we examine the bacterial ecology of supragingival plaque from four quadrants of the mouth along with a tongue swab from 26 healthy volunteers from South Florida (131 total sequences after filtering). As an area known to be a unique amalgamation of diverse cultures from across the globe, South Florida allows us to address the question of how supragingival plaque microbes differ across ethnic groups, thus potentially impacting treatment regiments related to oral issues. We assess overall phylogenetic abundance, alpha and beta diversity, and linear discriminate analysis of participants based on sex, ethnicity, sampling location in the mouth, and gingival health. Within this cohort, we find the presence of common phyla such as Firmicutes and common genera such as Streptococcus. Additionally, we find significant differences across sampling locations, sex, and gingival health. This research stresses the need for the continued incorporation of diverse populations within human oral microbiome studies.
数以万亿计的微生物构成了人类口腔，它们共同发挥着人体另一器官的作用。尽管该领域的研究已有数十年，但对于美国的西班牙裔、黑人及亚裔等代表性不足群体的口腔微生物群有何不同，尚无定论。在此，我们利用16S核糖体RNA测序技术，对来自南佛罗里达的26名健康志愿者口腔四个象限的龈上菌斑以及舌拭子进行细菌生态学研究（过滤后共获得131条序列）。南佛罗里达作为一个融合了全球多元文化的独特地区，使我们能够探讨龈上菌斑微生物在不同种族间的差异，进而可能影响与口腔问题相关的治疗方案。我们基于性别、种族、口腔采样位置和牙龈健康状况，评估参与者的总体系统发育丰度、α和β多样性以及线性判别分析。在这个队列中，我们发现了厚壁菌门等常见菌门以及链球菌属等常见菌属。此外，我们还发现不同采样位置、性别和牙龈健康状况之间存在显著差异。这项研究强调了在人类口腔微生物组研究中持续纳入不同人群的必要性。
