活血解毒化瘀方改善单侧输尿管梗阻大鼠对侧肾脏系膜细胞焦亡

Huoxue Jiedu Huayu Recipe Ameliorates Mesangial Cell Pyroptosis in Contralateral Kidney of UUO Rats.

作者信息

Zhang Yuxuan, Hao Juan, Ma Xuelian, Zhao Qiyue, Gao Xiaomeng, Wang Xiangting, Xu Qingyou

机构信息

Graduate School, Hebei University of Chinese Medicine, Shijiazhuang, China.

Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, Hebei University of Chinese Medicine, Shijiazhuang, China.

出版信息

Evid Based Complement Alternat Med. 2020 Dec 29;2020:2530431. doi: 10.1155/2020/2530431. eCollection 2020.

OBJECTIVES

To observe the effects of the Huoxue Jiedu Huayu Recipe (HJHR) on pyroptosis of glomerular mesangial cells in the contralateral unobstructed kidney (CK) of unilateral ureteral obstruction (UUO) rats.

METHODS

Sprague-Dawley rats were randomly divided into 4 groups: sham group, UUO group (10 days of left ureter ligation), UUO treated with eplerenone (EPL) (UUO + EPL) group, and UUO treated with HJHR (UUO + HJHR) group. The CKs of all rats were collected for studies.

RESULTS

Cell pyroptosis and macrophage infiltration was found in contralateral glomeruli, and nucleotide-binding oligomerization domain-like pyrin domain containing protein 3 (NLRP3) and interleukin (IL)-1 expression was upregulated in the CK of UUO rats. All of these changes were inhibited by HJHR and eplerenone. To determine how aldosterone (Aldo) activated the mineralocorticoid receptor (MR) and then induced mesangial cell pyroptosis with NLRP3-caspase-1-IL-1 pathway, human mesangial cells (HMCs) were treated with HJHR and eplerenone, which were examined to detect the expression of NLRP3 inflammasome-associated proteins following treatment with Aldo.

CONCLUSION

These results suggest that HJHR and eplerenone suppressed HMC pyroptosis via the MR/NLRP3 pathway.

目的

观察活血解毒化瘀方（HJHR）对单侧输尿管梗阻（UUO）大鼠对侧未梗阻肾脏（CK）肾小球系膜细胞焦亡的影响。

方法

将Sprague-Dawley大鼠随机分为4组：假手术组、UUO组（左侧输尿管结扎10天）、依普利酮（EPL）治疗的UUO组（UUO + EPL组）和HJHR治疗的UUO组（UUO + HJHR组）。收集所有大鼠的CK进行研究。

结果

在对侧肾小球中发现细胞焦亡和巨噬细胞浸润，并且在UUO大鼠的CK中含核苷酸结合寡聚化结构域样吡啉结构域蛋白3（NLRP3）和白细胞介素（IL）-1表达上调。所有这些变化均被HJHR和依普利酮抑制。为了确定醛固酮（Aldo）如何激活盐皮质激素受体（MR），然后通过NLRP3-半胱天冬酶-1-IL-1途径诱导系膜细胞焦亡，用人系膜细胞（HMCs）进行HJHR和依普利酮处理，在用Aldo处理后检测NLRP3炎性小体相关蛋白的表达。