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摄入细胞外囊泡增强了酪蛋白的摄取和疾病修饰的生物活性。

Casein-enhanced uptake and disease-modifying bioactivity of ingested extracellular vesicles.

机构信息

Cedars-Sinai Medical Center Smidt Heart Institute Los Angeles California USA.

出版信息

J Extracell Vesicles. 2021 Jan;10(3):e12045. doi: 10.1002/jev2.12045. Epub 2021 Jan 11.

DOI:10.1002/jev2.12045
PMID:33456725
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7798403/
Abstract

Extracellular vesicles (EVs) from cardiac stromal cells, developed as therapeutic candidates, improve dystrophic muscle function when administered parenterally, but oral delivery remains untested. We find that casein, the dominant protein in breast milk, enhances the uptake and bioactivity of ingested heart-derived EVs, altering gene expression in blood cells and enhancing muscle function in mice with muscular dystrophy. Thus, EVs, administered orally, are absorbed and exert disease-modifying bioactivity . Formulating EVs with casein enhances uptake and markedly expands the range of potential therapeutic applications.

摘要

细胞外囊泡（EVs）来源于心脏基质细胞，作为治疗候选物，当通过肠胃外途径给药时可改善营养不良肌肉的功能，但口服给药尚未经过测试。我们发现，乳清蛋白是母乳中的主要蛋白质，可增强摄入的心脏来源的 EVs 的摄取和生物活性，改变血细胞中的基因表达，并增强肌肉营养不良小鼠的肌肉功能。因此，口服给予 EVs 被吸收并发挥疾病修饰的生物活性。用乳清蛋白配制 EVs 可增强摄取并显著扩大潜在治疗应用的范围。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2601/7798403/37fd76aac339/JEV2-10-e12045-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2601/7798403/ebcdf6416f4f/JEV2-10-e12045-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2601/7798403/415f5ec0e95e/JEV2-10-e12045-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2601/7798403/daf2cf55ed77/JEV2-10-e12045-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2601/7798403/37fd76aac339/JEV2-10-e12045-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2601/7798403/ebcdf6416f4f/JEV2-10-e12045-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2601/7798403/415f5ec0e95e/JEV2-10-e12045-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2601/7798403/daf2cf55ed77/JEV2-10-e12045-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2601/7798403/37fd76aac339/JEV2-10-e12045-g004.jpg

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本文引用的文献

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JCI Insight. 2019 Apr 4;4(7). doi: 10.1172/jci.insight.125754.
2
A mechanistic roadmap for the clinical application of cardiac cell therapies.心脏细胞疗法临床应用的机制路线图。
Nat Biomed Eng. 2018 Jun;2(6):353-361. doi: 10.1038/s41551-018-0216-z. Epub 2018 Jun 11.
3
Identification of exosome-like nanoparticle-derived microRNAs from 11 edible fruits and vegetables.
Biomolecules. 2024 Jul 8;14(7):810. doi: 10.3390/biom14070810.
4
Large-scale production of extracellular vesicles: Report on the "massivEVs" ISEV workshop.细胞外囊泡的大规模生产：“massivEVs”国际细胞外囊泡学会研讨会报告
J Extracell Biol. 2022 Oct 25;1(10):e63. doi: 10.1002/jex2.63. eCollection 2022 Oct.
5
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bioRxiv. 2024 Apr 29:2024.04.27.591474. doi: 10.1101/2024.04.27.591474.
6
Unraveling the Multifaceted Roles of Extracellular Vesicles: Insights into Biology, Pharmacology, and Pharmaceutical Applications for Drug Delivery.解析细胞外囊泡的多面角色：在生物学、药理学和药物递送的药物应用方面的深入见解。
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7
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8
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6
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