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用于先进治疗的乳外泌体的分离和表征。

Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies.

机构信息

Instituto de Investigaciones Sanitarias de Aragón (IIS Aragón), 50009 Zaragoza, Spain.

Instituto Aragonés de Ciencias de la Salud (IACS), 50009 Zaragoza, Spain.

出版信息

Biomolecules. 2024 Jul 8;14(7):810. doi: 10.3390/biom14070810.

DOI:10.3390/biom14070810
PMID:39062527
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11275001/
Abstract

Exosomes are cell-derived extracellular vesicles (EVs) with diameters between 30 and 120 nm. In recent years, several studies have evaluated the therapeutic potential of exosomes derived from different fluids due to their low immunogenicity and high biocompatibility. However, producing exosomes on a large scale is still challenging. One of the fluids from which they could be isolated in large quantities is milk. Moreover, regeneration is a well-known property of milk. The present work seeks to optimize a method for isolating exosomes from bovine and human milk, comparing different storage conditions and different extraction protocols. We found differences in the yield extraction associated with pre-storage milk conditions and observed some differences according to the processing agent. When we removed milk fat globules and added rennet before freezing, we obtained a cleaner final fraction. In summary, we attempted to optimize a rennet-based new milk-exosome isolation method and concluded that pre-treatment, followed by freezing of samples, yielded the best exosome population.

摘要

外泌体是直径在 30 至 120nm 之间的细胞来源的细胞外囊泡 (EVs)。近年来，由于其免疫原性低、生物相容性高，许多研究评估了源自不同液体的外泌体的治疗潜力。然而，大规模生产外泌体仍然具有挑战性。其中一种可以大量分离外泌体的液体是牛奶。此外，牛奶具有再生功能。本研究旨在优化从牛和人乳中分离外泌体的方法，比较不同的储存条件和不同的提取方案。我们发现与储存前牛奶条件相关的提取产量存在差异，并根据处理剂观察到一些差异。当我们在冷冻前去除乳脂肪球并添加凝乳酶时，我们得到了更干净的最终级分。总之，我们尝试优化一种基于凝乳酶的新牛奶外泌体分离方法，得出的结论是，预处理后再冷冻样品可以获得最佳的外泌体群体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/784793bce9f9/biomolecules-14-00810-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/53a44de3c7a6/biomolecules-14-00810-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/a3398ba03e3e/biomolecules-14-00810-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/905ec9252e46/biomolecules-14-00810-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/944430ddf7f8/biomolecules-14-00810-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/eff20fba386a/biomolecules-14-00810-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/ce4c3fa400fb/biomolecules-14-00810-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/0f455248dcd0/biomolecules-14-00810-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/784793bce9f9/biomolecules-14-00810-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/53a44de3c7a6/biomolecules-14-00810-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/a3398ba03e3e/biomolecules-14-00810-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/905ec9252e46/biomolecules-14-00810-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/944430ddf7f8/biomolecules-14-00810-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/eff20fba386a/biomolecules-14-00810-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/ce4c3fa400fb/biomolecules-14-00810-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/0f455248dcd0/biomolecules-14-00810-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c990/11275001/784793bce9f9/biomolecules-14-00810-g007.jpg

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Plast Reconstr Surg Glob Open. 2022 Sep 14;10(9):e4505. doi: 10.1097/GOX.0000000000004505. eCollection 2022 Sep.
3
Milk-derived small extracellular vesicles: nanomaterials to promote bone formation.乳源小细胞外囊泡：促进骨形成的纳米材料。
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Adv Nutr. 2025 Jun;16(6):100430. doi: 10.1016/j.advnut.2025.100430. Epub 2025 Apr 25.
4
Exosomes in Breast Milk: Their Impact on the Intestinal Microbiota of the Newborn and Therapeutic Perspectives for High-Risk Neonates.母乳中的外泌体：它们对新生儿肠道微生物群的影响及高危新生儿的治疗前景。
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