Shang Fangjian, Jiang Xia, Wang Haobo, Chen Shihao, Wang Xin, Liu Ying, Guo Shang, Li Dongyun, Yu Weifang, Zhao Zengren, Wang Guiqi
Department of General Surgery, Hebei Key Laboratory of Colorectal Cancer Precision Diagnosis and Treatment, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Department of Pathology, The First Hospital of Hebei Medical University, Shijiazhuang, China.
J Gastrointest Oncol. 2020 Dec;11(6):1224-1232. doi: 10.21037/jgo-20-573.
Colorectal cancer (CRC) is the most common gastrointestinal malignancy. And probiotics may have the function of preventing colon cancer. The aim of this study was to investigate the inhibitory effects of a probiotic mixture on colorectal cancer and its potential mechanisms.
The effects of the probiotic mixture on proliferation and metastasis of mouse colon cancer CT26 cells were assessed by probiotics and cells co-culture assay, Cell Counting Kit-8 assay, colony formation assay, wound-healing assay, as well as migration and invasion assays. And CT26 cells were also transplanted into BALB/c mice to construct transplanted tumor animal model. The mice were randomly divided into two groups, control group and probiotic mixture intragastric administration group, after injection 21 days the tumor size and infiltration of immune cells in the tumor or spleen tissues were analyzed by hematoxylin and eosin (HE) and immunohistochemistry (IHC) staining.
The probiotic mixture significantly inhibited the proliferation, invasion, and migration ability of CT26 cells compare to the control cells (P<0.05). In the animal experiments, the tumor volume of mice that had been fed the probiotic mixture was significantly smaller than that of the control group (P<0.05). Compared with control mice, more apoptotic cells and infiltration of immune cells were showed in the tumor tissues of the mice treated with the probiotic mixture, and an increased number of CD8+ cells in the tumor and spleen tissues but no significant change in tissues.
These results suggested that the probiotic mixture could inhibit the growth of CT26 tumors and induce an immune response . The probiotic mixture also inhibited the invasion, migration, and proliferation ability of CT26 cells .
结直肠癌（CRC）是最常见的胃肠道恶性肿瘤。益生菌可能具有预防结肠癌的功能。本研究旨在探讨一种益生菌混合物对结直肠癌的抑制作用及其潜在机制。
通过益生菌与细胞共培养试验、细胞计数试剂盒-8试验、集落形成试验、伤口愈合试验以及迁移和侵袭试验，评估益生菌混合物对小鼠结肠癌CT26细胞增殖和转移的影响。将CT26细胞移植到BALB/c小鼠体内构建移植瘤动物模型。小鼠随机分为两组，对照组和益生菌混合物灌胃给药组，注射21天后，通过苏木精和伊红（HE）染色及免疫组织化学（IHC）染色分析肿瘤大小以及肿瘤或脾脏组织中免疫细胞的浸润情况。
与对照细胞相比，益生菌混合物显著抑制了CT26细胞的增殖、侵袭和迁移能力（P<0.05）。在动物实验中，喂食益生菌混合物的小鼠肿瘤体积明显小于对照组（P<0.05）。与对照小鼠相比，用益生菌混合物处理的小鼠肿瘤组织中显示出更多的凋亡细胞和免疫细胞浸润，肿瘤和脾脏组织中CD8+细胞数量增加，但组织中无显著变化。
这些结果表明，益生菌混合物可以抑制CT26肿瘤的生长并诱导免疫反应。益生菌混合物还抑制了CT26细胞的侵袭、迁移和增殖能力。
