高血压相关肾细胞癌潜在生物标志物和治疗靶点的生物信息学基因分析
Bioinformatic gene analysis for possible biomarkers and therapeutic targets of hypertension-related renal cell carcinoma.
作者信息
Huang Wenjie, Wu Ke, Wu Ruoyu, Chen Zhiguo, Zhai Wei, Zheng Junhua
机构信息
Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Urology, Renji Hospital, School of Medicine in Shanghai Jiao Tong University, Shanghai, China.
出版信息
Transl Androl Urol. 2020 Dec;9(6):2675-2687. doi: 10.21037/tau-20-817.
BACKGROUND
Renal cell carcinoma (RCC) is one of the most prevalent malignant tumors of the urinary system. Hypertension can cause hypertensive nephropathy (HN). Meanwhile, Hypertension is considered to be related to kidney cancer. We analyzed co-expressed genes to find out the relationship between hypertension and RCC and show possible biomarkers and novel therapeutic targets of hypertension-related RCC.
METHODS
We identified the differentially expressed genes (DEGs) of HN and RCC through analyzing Gene Expression Omnibus (GEO) datasets GSE99339, GSE99325, GSE53757 and GSE15641 by means of bioinformatics analysis, respectively. Then we evaluated these genes with protein-protein interaction (PPI) networks, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and CTD database. Subsequently, we verified co-expressed DEGs with Gene Expression Profiling Interactive Analysis (GEPIA) database. Finally, corresponding predicted miRNAs of co-expressed DEGs were identified and verified via mirDIP database and Starbase, respectively.
RESULTS
We identified 9 co-expressed DEGs, including , , , , , , , , and . and and their corresponding predicted miRNAs, especially hsa-miR-221-5p, hsa-miR-205-5p, hsa-miR-152-3p and hsa-miR-137 may be notably related to hypertension-related RCC.
CONCLUSIONS
and genes have great potential to become novel biomarkers and therapeutic targets concerning hypertension-related RCC.
背景
肾细胞癌（RCC）是泌尿系统最常见的恶性肿瘤之一。高血压可导致高血压肾病（HN）。同时，高血压被认为与肾癌有关。我们分析共表达基因以找出高血压与RCC之间的关系，并展示高血压相关RCC可能的生物标志物和新的治疗靶点。
方法
我们分别通过生物信息学分析，分析基因表达综合数据库（GEO）数据集GSE99339、GSE99325、GSE53757和GSE15641，确定HN和RCC的差异表达基因（DEGs）。然后我们通过蛋白质-蛋白质相互作用（PPI）网络、基因本体论（GO）分析、京都基因与基因组百科全书（KEGG）通路分析和CTD数据库对这些基因进行评估。随后，我们使用基因表达谱交互式分析（GEPIA）数据库验证共表达的DEGs。最后，分别通过mirDIP数据库和Starbase鉴定并验证共表达DEGs的相应预测miRNA。
结果
我们鉴定出9个共表达的DEGs，包括 、 、 、 、 、 、 、 和 。 、 及其相应的预测miRNA，尤其是hsa-miR-221-5p、hsa-miR-205-5p、hsa-miR-152-3p和hsa-miR-137可能与高血压相关RCC显著相关。
结论
和 基因有很大潜力成为与高血压相关RCC的新型生物标志物和治疗靶点。
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