Agrawal Ishan, Sharma Nidhi, Saxena Shivanjali, Arvind S, Chakraborty Debayani, Chakraborty Debarati Bhunia, Jha Deepak, Ghatak Surajit, Epari Sridhar, Gupta Tejpal, Jha Sushmita
Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India.
Karolinska Institute and Scilifelab, Stockholm, Sweden.
iScience. 2021 Jan 6;24(1):101968. doi: 10.1016/j.isci.2020.101968. eCollection 2021 Jan 22.
Dopamine (DA) plays many roles in the brain, especially in movement, motivation, and reinforcement of behavior; however, its role in regulating innate immunity is not clear. Here, we show that DA can induce DNA-based extracellular traps in primary, adult, human microglia and BV2 microglia cell line. These DNA-based extracellular traps are formed independent of reactive oxygen species, actin polymerization, and cell death. These traps are functional and capture fluorescein (FITC)-tagged even when reactive oxygen species production or actin polymerization is inhibited. We show that microglial extracellular traps are present in . This is crucial because cells are known to secrete DA. Our findings demonstrate that DA plays a significant role in sterile neuro-inflammation by inducing microglia extracellular traps.
多巴胺（DA）在大脑中发挥着多种作用，尤其是在运动、动机和行为强化方面；然而，其在调节固有免疫中的作用尚不清楚。在此，我们表明多巴胺可在原代成人人类小胶质细胞和BV2小胶质细胞系中诱导基于DNA的细胞外陷阱形成。这些基于DNA的细胞外陷阱的形成独立于活性氧、肌动蛋白聚合和细胞死亡。即使活性氧生成或肌动蛋白聚合受到抑制，这些陷阱仍具有功能并能捕获荧光素（FITC）标记物。我们发现小胶质细胞外陷阱存在于……。这至关重要，因为已知……细胞会分泌多巴胺。我们的研究结果表明，多巴胺通过诱导小胶质细胞外陷阱在无菌性神经炎症中发挥重要作用。
