通过Seahorse XF96细胞线粒体应激试验测量贴壁细胞中的线粒体呼吸。
Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test.
作者信息
Gu Xia, Ma Yuan, Liu Yi, Wan Qiang
机构信息
School of Medicine, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China.
Renal Division, Peking University First Hospital, Peking University Institute of Nephrology, Beijing 100034, China.
出版信息
STAR Protoc. 2020 Dec 30;2(1):100245. doi: 10.1016/j.xpro.2020.100245. eCollection 2021 Mar 19.
Mitochondria play pivotal roles in cellular energy metabolism. Most of the intracellular adenosine triphosphate (ATP) is generated by mitochondrial respiration. The Cell Mito Stress Test is a common method to measure the key parameters of mitochondrial respiration. Here, we use the human cell line HK-2 as an example to present the procedures to quantify the oxygen consumption rate using a Seahorse XFe96 extracellular flux analyzer. For complete details on the use and execution of this protocol, please refer to Ma et al. (2020).
线粒体在细胞能量代谢中发挥着关键作用。细胞内的三磷酸腺苷（ATP）大部分是通过线粒体呼吸产生的。细胞线粒体应激试验是一种测量线粒体呼吸关键参数的常用方法。在此，我们以人肾近端小管上皮细胞系HK-2为例，介绍使用海马XFe96细胞外通量分析仪定量测定氧消耗率的步骤。有关本方案使用和执行的完整详细信息，请参考Ma等人（2020年）的文献。
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