Shiseido Global Innovation Center, 1-2-11, Takashima, Nishi-ku, Yokohama, Kanagawa 220-0011, Japan.
Analyst. 2021 Feb 21;146(4):1142-1150. doi: 10.1039/d0an02039g. Epub 2021 Jan 18.
Many dermatological studies have had limited success in revealing skin function because conventional histological methods are known to affect skin components. Recent progress in non-invasive optical imaging has enabled non-invasive visualization of the structure of each skin layer. However, it remains difficult to identify individual skin components. Alternatively, it is possible to obtain molecular vibrational signatures using spontaneous Raman scattering microscopy. Spontaneous Raman scattering microscopy requires long acquisition times and is rarely applied to skin imaging, especially because skin components, such as water and transepidermal agents, undergo relatively rapid changes. Consequently, non-linear Raman microscopies, such as coherent anti-Stokes Raman scattering and stimulated Raman scattering, have gradually been applied to acquire molecular imaging of skin tissue. In this review, the applications of Raman microscopies used to evaluate skin and research trends are presented. The applications of spontaneous Raman microscopy to in vivo human skin evaluation are first demonstrated with typical applications. Finally, the latest application of coherent Raman scattering microscopy to visualize 3D intracellular morphologies in the human epidermis during differentiation is described.
许多皮肤科研究在揭示皮肤功能方面收效甚微，因为众所周知，传统的组织学方法会影响皮肤成分。最近非侵入性光学成像的进展使得对每个皮肤层结构的非侵入性可视化成为可能。然而，仍然难以识别单个皮肤成分。或者，可以使用自发拉曼散射显微镜获得分子振动特征。自发拉曼散射显微镜需要较长的采集时间，很少应用于皮肤成像，特别是因为皮肤成分，如水和经皮试剂，会经历相对较快的变化。因此，诸如相干反斯托克斯拉曼散射和受激拉曼散射之类的非线性拉曼显微镜已逐渐被应用于获取皮肤组织的分子成像。在这篇综述中，介绍了用于评估皮肤的拉曼显微镜的应用和研究趋势。首先用典型的应用展示了自发拉曼显微镜在体内人皮肤评估中的应用。最后，描述了相干拉曼散射显微镜在分化过程中可视化人表皮 3D 细胞内形态的最新应用。
