志贺氏菌在基本培养基中生长的营养需求。
Nutritional requirements of shigellae for growth in a minimal medium.
作者信息
Ahmed Z U, Sarker M R, Sack D A
机构信息
International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
出版信息
Infect Immun. 1988 Apr;56(4):1007-9. doi: 10.1128/iai.56.4.1007-1009.1988.
Most (about 81%) of the clinical isolates of shigellae that were tested failed to grow in a minimal medium. Of the auxotrophic isolates belonging to the four Shigella species, 98% grew in a minimal medium containing methionine, nicotinic acid, and tryptophan. The combination of methionine and tryptophan appears to be an obligatory requirement for Shigella dysenteriae serotype 1 strains, while the combination of nicotinic acid and tryptophan appears to be obligatory for serotype 2. Requirements which varied in other isolates were, however, genetically stable, indicating that the auxotypes may be useful as epidemiological markers. Cultures of shigellae in liquid minimal medium containing the above three supplements showed rapid growth and gave reasonably high cell yields.
大多数（约81%）接受检测的志贺氏菌临床分离株无法在基本培养基中生长。在属于四种志贺氏菌属的营养缺陷型分离株中，98%能在含有蛋氨酸、烟酸和色氨酸的基本培养基中生长。蛋氨酸和色氨酸的组合似乎是痢疾志贺氏菌1型菌株的必需成分，而烟酸和色氨酸的组合似乎是2型菌株的必需成分。然而，其他分离株中不同的需求在遗传上是稳定的，这表明营养缺陷型可能作为流行病学标记物有用。在含有上述三种补充剂的液体基本培养基中培养志贺氏菌显示生长迅速且细胞产量相当高。
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