拟南芥的雄性育性需要控制花粉管功能的基因的活性 DNA 去甲基化。
Male fertility in Arabidopsis requires active DNA demethylation of genes that control pollen tube function.
机构信息
Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Ecole Normale Supérieure, PSL Research University, 75005, Paris, France.
Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, NY, 11724, USA.
出版信息
Nat Commun. 2021 Jan 18;12(1):410. doi: 10.1038/s41467-020-20606-1.
Active DNA demethylation is required for sexual reproduction in plants but the molecular determinants underlying this epigenetic control are not known. Here, we show in Arabidopsis thaliana that the DNA glycosylases DEMETER (DME) and REPRESSOR OF SILENCING 1 (ROS1) act semi-redundantly in the vegetative cell of pollen to demethylate DNA and ensure proper pollen tube progression. Moreover, we identify six pollen-specific genes with increased DNA methylation as well as reduced expression in dme and dme;ros1. We further show that for four of these genes, reinstalling their expression individually in mutant pollen is sufficient to improve male fertility. Our findings demonstrate an essential role of active DNA demethylation in regulating genes involved in pollen function.
植物的有性生殖需要活性 DNA 去甲基化，但这种表观遗传控制的分子决定因素尚不清楚。在这里，我们在拟南芥中表明，DNA 糖苷酶 DEMETER（DME）和沉默抑制物 1（ROS1）在花粉的营养细胞中半冗余地发挥作用，以去甲基化 DNA 并确保花粉管的正常发育。此外，我们还鉴定了六个花粉特异性基因，这些基因在 dme 和 dme;ros1 中表现出 DNA 甲基化增加和表达减少。我们进一步表明，对于其中的四个基因，单独在突变花粉中重新表达它们的表达足以提高雄性育性。我们的研究结果表明，活性 DNA 去甲基化在调节参与花粉功能的基因方面起着重要作用。
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