Liao Tzu-Ying, Easton Christopher D, Thissen Helmut, Tsai Wei-Bor
Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan.
CSIRO Manufacturing, Research Way, Clayton 3168, Victoria, Australia.
ACS Biomater Sci Eng. 2020 Jun 8;6(6):3349-3360. doi: 10.1021/acsbiomaterials.0c00148. Epub 2020 May 19.
Medical device associated infections remain a significant problem for all classes of devices at this point in time. Here, we have developed a surface modification technique to fabricate multifunctional coatings that combine antifouling and antimicrobial properties. Zwitterionic polymers providing antifouling properties and quaternary ammonium containing polymers providing antimicrobial properties were combined in these coatings. Throughout this study, aminomalononitrile (AMN) was used to achieve one-step coatings incorporating different polymers. The characterization of coatings was carried out using static water contact angle (WCA) measurements, X-ray photoelectron spectroscopy (XPS), profilometry, and scanning electron microscopy (SEM), whereas the biological response in vitro was analyzed using and as well as L929 fibroblast cells. Zwitterionic polymers synthesized from sulfobetaine methacrylate and 2-aminoethyl methacrylate were demonstrated to reduce bacterial attachment when incorporated in AMN assisted coatings. However, bacteria in suspension were not affected by this approach. On the other hand, alkylated polyethylenimine polymers, synthesized to provide quaternary ammonium groups, were demonstrated to have contact killing properties when incorporated in AMN assisted coatings. However, the high bacterial attachment observed on these surfaces may be detrimental in applications requiring longer-term bactericidal activity. Therefore, AMN-assisted coatings containing both quaternary and zwitterionic polymers were fabricated. These multifunctional coatings were demonstrated to significantly reduce the number of live bacteria not only on the modified surfaces, but also in suspension. This approach is expected to be of interest in a range of biomedical device applications.
在当前，医疗设备相关感染对于所有类型的设备而言仍然是一个重大问题。在此，我们开发了一种表面改性技术来制备兼具防污和抗菌性能的多功能涂层。两性离子聚合物提供防污性能，含季铵盐的聚合物提供抗菌性能，二者被结合在这些涂层中。在整个研究过程中，氨基丙二腈（AMN）被用于实现包含不同聚合物的一步法涂层。使用静态水接触角（WCA）测量、X射线光电子能谱（XPS）、轮廓仪和扫描电子显微镜（SEM）对涂层进行表征，而使用[具体实验方法1]和[具体实验方法2]以及L929成纤维细胞分析体外生物反应。由甲基丙烯酰基磺酸甜菜碱和甲基丙烯酸2-氨基乙酯合成的两性离子聚合物在掺入AMN辅助涂层时被证明可减少细菌附着。然而，悬浮液中的细菌不受此方法影响。另一方面，合成的用于提供季铵基团的烷基化聚乙烯亚胺聚合物在掺入AMN辅助涂层时被证明具有接触杀灭性能。然而，在这些表面上观察到的高细菌附着在需要长期杀菌活性的应用中可能是有害的。因此，制备了同时含有季铵和两性离子聚合物的AMN辅助涂层。这些多功能涂层不仅在改性表面上，而且在悬浮液中都被证明能显著减少活菌数量。这种方法有望在一系列生物医学设备应用中受到关注。
