Vadivelmurugan Adhisankar, Sharmila Ramalingam, Pan Whei-Lin, Tsai Shiao-Wen
Department of Biomedical Engineering, Chang Gung University, Taoyuan 33302, Taiwan.
Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 300044, Taiwan.
ACS Omega. 2023 Oct 26;8(44):41909-41917. doi: 10.1021/acsomega.3c06991. eCollection 2023 Nov 7.
After orthopedic surgery, antibiotics are usually employed to reduce the risk of infection. If it is possible to enhance antimicrobial functionality and incorporate antimicrobial agents into the bone-filling matrix, not only it can promote bone tissue regeneration, but it can also enable localized administration of medication to elevate antibacterial efficacy. Meanwhile, previous studies have shown that calcium and strontium can support the growth of osteoblastic cells and diminish bone resorption or deterioration. In the past few years, metal-organic frameworks (MOFs) have been widely used as drug carriers owing to their characteristic advantages. In this study, a MOF was prepared in an aqueous solution by a simple coprecipitation method with the organic ligand 1,3,5-tricarboxylic benzene (HBTC) as a linker to form Ca-Sr-MOF. Furthermore, the Ca-Sr-MOF was coated with aminomalononitrile (AMN), which adhered through the electrostatic interactions between HBTC and AMN. With this MOF (Ca-Sr-AMN-MOF), AMN polymerization reactions can occur in aqueous environments, and a polymer layer was observed on the MOF surface with moderate hydrophilicity. The prepared Ca-Sr-MOF and Ca-Sr-AMN-MOF were characterized by Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy, high-resolution transmission electron microscopy, and UV-visible spectroscopy. Finally, tetracycline (TC) was selected as the model drug to measure the drug loading efficiency, release profile, and antibiotic activity. The percent cumulative drug release of TC from Ca-Sr-MOF and Ca-Sr-AMN-MOF was 55.15 and 9.1%, respectively. The antibacterial effectiveness of TC-loaded MOF against Gram-negative bacteria was evaluated, revealing the remarkable antimicrobial performance of these substances.
骨科手术后，通常会使用抗生素来降低感染风险。如果能够增强抗菌功能并将抗菌剂纳入骨填充基质中，不仅可以促进骨组织再生，还能够实现局部给药以提高抗菌效果。同时，先前的研究表明，钙和锶可以支持成骨细胞的生长，并减少骨吸收或骨退化。在过去几年中，金属有机框架（MOF）因其独特的优势而被广泛用作药物载体。在本研究中，通过简单的共沉淀法，以有机配体1,3,5-苯三甲酸（HBTC）作为连接体，在水溶液中制备了一种MOF，形成Ca-Sr-MOF。此外，用氨基丙二腈（AMN）包覆Ca-Sr-MOF，AMN通过HBTC与AMN之间的静电相互作用附着。利用这种MOF（Ca-Sr-AMN-MOF），AMN聚合反应可以在水性环境中发生，并且在MOF表面观察到具有适度亲水性的聚合物层。通过傅里叶变换红外光谱、粉末X射线衍射、扫描电子显微镜、高分辨率透射电子显微镜和紫外可见光谱对制备的Ca-Sr-MOF和Ca-Sr-AMN-MOF进行了表征。最后，选择四环素（TC）作为模型药物来测量载药效率、释放曲线和抗生素活性。TC从Ca-Sr-MOF和Ca-Sr-AMN-MOF中的累积药物释放百分比分别为55.15%和9.1%。评估了载有TC的MOF对革兰氏阴性菌的抗菌效果，揭示了这些物质显著的抗菌性能。
