Wang Wei, Liu Yang, Yang Chao, Jia Weitao, Qi Xin, Liu Changsheng, Li Xiaolin
Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China.
ACS Biomater Sci Eng. 2020 Jun 8;6(6):3539-3549. doi: 10.1021/acsbiomaterials.0c00558. Epub 2020 May 8.
The efficiency of drugs often hinges on drug carriers. To effectively transport therapeutic plant molecules, drug delivery carriers should be able to carry large doses of therapeutic drugs, enable their sustained release, and maintain their biological activity. Here, graphene oxide (GO) is demonstrated to be a valid carrier for delivering therapeutic plant molecules. Salvianolic acid B (SB), which contains a large number of hydroxyl groups, bound to the carboxyl groups of GO by self-assembly. Silk fibroin (SF) substrates were combined with functionalized GO through the freeze-drying method. SF/GO scaffolds could be loaded with large doses of SB, maintain the biological activity of SB while continuously releasing SB, and significantly promote the osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs). SF/GO/SB also dramatically enhanced endothelial cell (EA-hy9.26) migration and tubulogenesis in vitro. Eight weeks after implantation of SF/GO/SB scaffolds in a rat cranial defect model, the defect area showed more new bone and angiogenesis than that following SF and SF/GO scaffold implantation. Therefore, GO is an effective sustained-release carrier for therapeutic plant molecules, such as SB, which can repair bone defects by promoting osteogenic differentiation and angiogenesis.
药物的疗效通常取决于药物载体。为了有效地运输治疗性植物分子，药物递送载体应能够携带大剂量的治疗药物，实现其持续释放，并保持其生物活性。在此，氧化石墨烯（GO）被证明是一种递送治疗性植物分子的有效载体。含有大量羟基的丹酚酸B（SB）通过自组装与GO的羧基结合。丝素蛋白（SF）基质通过冷冻干燥法与功能化的GO相结合。SF/GO支架可以负载大剂量的SB，在持续释放SB的同时保持SB的生物活性，并显著促进大鼠骨髓间充质干细胞（rBMSC）的成骨分化。SF/GO/SB在体外还显著增强了内皮细胞（EA-hy9.26）的迁移和血管生成。在大鼠颅骨缺损模型中植入SF/GO/SB支架八周后，缺损区域显示出比植入SF和SF/GO支架后更多的新骨形成和血管生成。因此，GO是一种用于治疗性植物分子（如SB）的有效缓释载体，其可以通过促进成骨分化和血管生成来修复骨缺损。
