Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, Doha-2713, Qatar.
Biomedical Research Centre, Qatar University, Doha-2713, Qatar.
ACS Biomater Sci Eng. 2020 Jan 13;6(1):58-70. doi: 10.1021/acsbiomaterials.8b01352. Epub 2019 Jul 23.
Insufficient cell proliferation, cell migration, and angiogenesis are among the major causes for nonhealing of chronic diabetic wounds. Incorporation of cerium oxide nanoparticles (nCeO) in wound dressings can be a promising approach to promote angiogenesis and healing of diabetic wounds. In this paper, we report the development of a novel nCeO containing electrospun poly(3-hydroxybutyrate--3-hydroxyvalerate) (PHBV) membrane for diabetic wound healing applications. cell adhesion studies, chicken embryo angiogenesis assay, and diabetic wound healing studies were performed to assess the cell proliferation, angiogenesis, and wound healing potential of the developed membranes. The experimental results showed that nCeO containing PHBV membranes can promote cell proliferation and cell adhesion when used as wound dressings. For less than 1% w/w of nCeO content, human mammary epithelial cells (HMEC) were adhered parallel to the individual fibers of PHBV. For higher than 1% w/w of nCeO content, cells started to flatten and spread over the fibers. angiogenic assay showed the ability of nCeO incorporated PHBV membranes to enhance blood vessel formation. wound healing study in diabetic rats confirmed the wound healing potential of nCeO incorporated PHBV membranes. The study suggests that nCeO incorporated PHBV membranes have strong potential to be used as wound dressings to enhance cell proliferation and vascularization and promote the healing of diabetic wounds.
细胞增殖、迁移和血管生成不足是慢性糖尿病伤口难以愈合的主要原因之一。将氧化铈纳米粒子 (nCeO) 掺入伤口敷料中可能是促进血管生成和糖尿病伤口愈合的一种有前途的方法。在本文中，我们报告了一种新型含 nCeO 的电纺聚(3-羟基丁酸酯-3-羟基戊酸酯) (PHBV) 膜的开发，用于糖尿病伤口愈合应用。进行了细胞粘附研究、鸡胚血管生成测定和糖尿病伤口愈合研究，以评估所开发膜的细胞增殖、血管生成和伤口愈合潜力。实验结果表明，用作伤口敷料时，含 nCeO 的 PHBV 膜可以促进细胞增殖和细胞粘附。当 nCeO 含量低于 1%w/w 时，人乳腺上皮细胞 (HMEC) 沿 PHBV 的单个纤维平行粘附。当 nCeO 含量高于 1%w/w 时，细胞开始在纤维上变平并扩散。血管生成测定表明，掺入 nCeO 的 PHBV 膜具有增强血管形成的能力。糖尿病大鼠的伤口愈合研究证实了掺入 nCeO 的 PHBV 膜的伤口愈合潜力。该研究表明，掺入 nCeO 的 PHBV 膜具有很大的潜力可用作伤口敷料，以增强细胞增殖和血管生成，促进糖尿病伤口的愈合。
