Le Yen-Chen, Su Wen-Ta
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1, Sec. 3, Chung-Hsiao E. Rd, Taipei 106344, Taiwan.
Int J Mol Sci. 2025 Jun 25;26(13):6087. doi: 10.3390/ijms26136087.
Wound healing is a complex biological process that benefits from advanced biomaterials capable of modulating inflammation and promoting tissue regeneration. In this study, cerium oxide nanoparticles (CeONPs) were green-synthesized using extract, which served as both a reducing and stabilizing agent. The CeONPs exhibited a spherical morphology, a face-centered cubic crystalline structure, and an average size of 9.39 nm, as confirmed by UV-Vis spectroscopy, FTIR, XRD, and TEM analyses. These nanoparticles demonstrated no cytotoxicity and promoted fibroblast migration, while significantly suppressing the production of inflammatory mediators (TNF-α, IL-6, iNOS, NO, and ROS) in LPS-stimulated RAW264.7 macrophages. Gene expression analysis indicated M2 macrophage polarization, with upregulation of Arg-1, IL-10, IL-4, and TGF-β. Aligned polycaprolactone/polylactic acid (PCL/PLA) nanofibers embedded with CeONPs were fabricated using electrospinning. The composite nanofibers exhibited desirable physicochemical properties, including porosity, mechanical strength, swelling behavior, and sustained cerium ions release. In a rat full-thickness wound model, the CeO nanofiber-treated group showed a 22% enhancement in wound closure compared to the control on day 11. Histological evaluation revealed reduced inflammation, enhanced granulation tissue, neovascularization, and increased collagen deposition. These results highlight the therapeutic potential of CeO-incorporated nanofiber scaffolds for accelerated wound repair and inflammation modulation.
伤口愈合是一个复杂的生物学过程，先进的生物材料能够调节炎症并促进组织再生，从而使伤口愈合受益。在本研究中，使用提取物绿色合成了氧化铈纳米颗粒（CeONPs），该提取物同时作为还原剂和稳定剂。紫外可见光谱、傅里叶变换红外光谱、X射线衍射和透射电子显微镜分析证实，CeONPs呈现球形形态、面心立方晶体结构，平均尺寸为9.39nm。这些纳米颗粒无细胞毒性，可促进成纤维细胞迁移，同时显著抑制脂多糖刺激的RAW264.7巨噬细胞中炎症介质（TNF-α、IL-6、iNOS、NO和ROS）的产生。基因表达分析表明M2巨噬细胞极化，Arg-1、IL-10、IL-4和TGF-β上调。采用静电纺丝法制备了嵌入CeONPs的取向聚己内酯/聚乳酸（PCL/PLA）纳米纤维。复合纳米纤维具有理想的物理化学性质，包括孔隙率、机械强度、溶胀行为和铈离子的持续释放。在大鼠全层伤口模型中，与对照组相比，CeO纳米纤维治疗组在第11天时伤口闭合率提高了22%。组织学评估显示炎症减轻、肉芽组织增强、新血管形成和胶原沉积增加。这些结果突出了含CeO纳米纤维支架在加速伤口修复和调节炎症方面的治疗潜力。
