Ataturk University, Faculty of Science, Department of Chemistry, 25240-, Erzurum, Turkey.
Erzincan University, Faculty of Science, Department of Chemistry, 24100, Erzincan, Turkey.
Chem Biodivers. 2021 Mar;18(3):e2000812. doi: 10.1002/cbdv.202000812. Epub 2021 Feb 8.
In this study, phenolic composition, and in vitro biological activities of ethyl acetate (EAE) and methanol (ME) extracts obtained from the aerial parts of endemic Tanacetum erzincanense were investigated. Total phenolic and flavonoid content of the extracts were determined by Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. Antioxidant capacity of the extracts was evaluated over radical scavenging (DPPH and ABTS) and metal ion reducing power (FRAP and CUPRAC) tests. Individual phenolic compounds in ME was analyzed by high-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF/MS). Cell inhibitory potential of the extracts was tested against colorectal adenocarcinoma (HT-29), breast adenocarcinoma (MCF-7), and hepatocarcinoma (HepG2) cells by 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. The results showed that ME contains higher TPC (64.4 mg GAE/g) and TFC (62.2 mg QE/g) than those of EAE (41.5 mg GAE/g and 40.0 mg QE/g). LC-ESI-QTOF/MS analysis revealed that ME is rich in phenolic compounds, namely, chlorogenic acid, apigenin, quercetin, luteolin, and diosmetin. Antioxidant assay results indicated that ME possess stronger activity than EAE and a power that competes with synthetic antioxidants. XTT assay results demonstrated that although both extracts displayed a considerable cytotoxicity against the tested cancer cell lines in a time and dose-dependent manner, ME expressed its selective inhibitory action towards MCF-7 cells with an IC value of 20.4 μg/mL for 72 h. These results may serve as a basis for further in vivo studies to examine the potential applications of T. erzincanense in food and pharmaceutical industries.
在这项研究中，研究了从地方性菊属植物的地上部分获得的乙酸乙酯（EAE）和甲醇（ME）提取物的酚类成分和体外生物活性。通过福林-考尔考特比色法和三氯化铝比色法分别测定提取物的总酚和类黄酮含量。通过自由基清除（DPPH 和 ABTS）和金属离子还原能力（FRAP 和 CUPRAC）试验评估提取物的抗氧化能力。通过高效液相色谱-电喷雾电离四极杆飞行时间质谱联用（LC-ESI-QTOF/MS）分析 ME 中的单个酚类化合物。通过 2,3-双（2-甲氧基-4-硝基-5-磺苯基）-2H-四唑-5-羧基苯胺（XTT）测定法测试提取物对结直肠腺癌（HT-29）、乳腺腺癌（MCF-7）和肝癌（HepG2）细胞的细胞抑制潜力。结果表明，ME 中的 TPC（64.4mgGAE/g）和 TFC（62.2mgQE/g）均高于 EAE（41.5mgGAE/g 和 40.0mgQE/g）。LC-ESI-QTOF/MS 分析表明，ME 富含酚类化合物，即绿原酸、芹菜素、槲皮素、木犀草素和染料木素。抗氧化测定结果表明，ME 比 EAE 具有更强的活性，其活性与合成抗氧化剂相当。XTT 测定结果表明，尽管两种提取物均以时间和剂量依赖的方式对测试的癌细胞系表现出相当大的细胞毒性，但 ME 对 MCF-7 细胞表现出选择性抑制作用，其 IC 值为 20.4μg/mL，作用 72h。这些结果可为进一步的体内研究提供依据，以研究 Erzincanense 在食品和制药行业的潜在应用。
