Abdel Fattah Abdel Rahman, Meleca Elvira, Mishriki Sarah, Lelic Alina, Geng Fei, Sahu Rakesh P, Ghosh Suvojit, Puri Ishwar K
Department of Mechanical Engineering, §School of Biomedical Engineering, ⊥McMaster Immunology Research Center, and ∥Department of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, OntarioL8S 4L7, Canada.
Department of Mechanical Engineering, School of Biomedical Engineering, ⊥McMaster Immunology Research Center, and ∥Department of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, OntarioL8S 4L7, Canada.
ACS Biomater Sci Eng. 2016 Dec 12;2(12):2133-2138. doi: 10.1021/acsbiomaterials.6b00614. Epub 2016 Nov 8.
Using whole blood, we demonstrate the first realization of a novel macroscale, contactless, label-free method to print in situ three-dimensional (3D) cell assemblies of different morphologies and sizes. This novel bioprinting method does not use nozzles that can contaminate the cell suspension, or to which cells can adhere. Instead, we utilize the intrinsic diamagnetic properties of whole blood cells to magnetically manipulate them in situ in a nontoxic paramagnetic medium, creating (a) rectangular bar, (b) three-pointed star, and (c) spheroids of varying sizes. We envision the technique to be transferable to other cell lines, with potential applications in tissue engineering and drug screening.
我们使用全血，首次实现了一种新型的宏观尺度、非接触、无标记方法，用于原位打印不同形态和大小的三维（3D）细胞聚集体。这种新型生物打印方法不使用可能污染细胞悬液或细胞可能附着的喷嘴。相反，我们利用全血细胞的固有抗磁性，在无毒顺磁性介质中原位对其进行磁操控，创建了（a）矩形条、（b）三星形和（c）不同大小的球体。我们设想该技术可转移到其他细胞系，在组织工程和药物筛选中具有潜在应用。
