Zhang Hualin, Wang Juan, Ma Hairong, Zhou Yueli, Ma Xuerong, Liu Jinsong, Huang Jin, Yu Na
College of Stomatology, Ningxia Medical University, Yinchuan 750004, China.
General Hospital of Ningxia Medical University, Yinchuan 750004, China.
ACS Biomater Sci Eng. 2016 Dec 12;2(12):2162-2175. doi: 10.1021/acsbiomaterials.6b00357. Epub 2016 Oct 24.
Regenerative procedures using guided tissue regeneration (GTR) membrane are presently well-established in periodontal therapy. In this study, bilayered poly(lactic--glycolic acid) (PLGA)/wool keratin (WK) composite membranes were prepared for use as GTR membrane by the solvent casting and electrospinning methods. Then, the composite membranes were used to evaluate the effects in guided tissue regeneration in beagle dogs. The results showed that the bottom layer (casting film) of the bilayered PLGA/wool keratin composite membranes was a compact PLGA/wool keratin membrane, and the upper layer (electrospun film) was a loose, porous, three-dimensional, and fibrous PLGA/wool keratin membrane (The wool keratin was at five different levels: 0, 0.5, 1, 2, or 4 wt %). The mechanical properties of the composites were significantly enhanced by the addition of wool keratin. The bilayered PLGA/1.0% wool keratin composite membranes presented the best values of ultimate strength and Young's modulus. All of the bilayered PLGA/wool keratin composite membranes showed high thermal and thermooxidative stabilities. The GTR results showed that the PLGA/1.0% wool keratin composite membranes could effectively promote the periodontal tissue regeneration after 12 weeks, and have a similar effect to the collagen membrane on the regeneration of the periodontal tissue. Thus, the bilayered PLGA/wool keratin composite membranes show great potential to meet the demand for GTR membrane. The study will serve as a foundation for further study of the PLGA/wool keratin membranes for GTR application and the therapy of periodontal disease.
目前，使用引导组织再生（GTR）膜的再生程序在牙周治疗中已得到广泛应用。在本研究中，通过溶剂浇铸和静电纺丝方法制备了双层聚乳酸-乙醇酸共聚物（PLGA）/羊毛角蛋白（WK）复合膜，用作GTR膜。然后，使用复合膜评估其在比格犬引导组织再生中的效果。结果表明，双层PLGA/羊毛角蛋白复合膜的底层（浇铸膜）是致密的PLGA/羊毛角蛋白膜，上层（静电纺丝膜）是疏松、多孔、三维的纤维状PLGA/羊毛角蛋白膜（羊毛角蛋白含量有五个不同水平：0、0.5、1、2或4 wt%）。添加羊毛角蛋白显著提高了复合材料的力学性能。双层PLGA/1.0%羊毛角蛋白复合膜的极限强度和杨氏模量值最佳。所有双层PLGA/羊毛角蛋白复合膜均表现出高的热稳定性和热氧化稳定性。GTR结果表明，PLGA/1.0%羊毛角蛋白复合膜在12周后能有效促进牙周组织再生，对牙周组织再生的效果与胶原膜相似。因此，双层PLGA/羊毛角蛋白复合膜在满足GTR膜需求方面具有巨大潜力。该研究将为进一步研究用于GTR应用的PLGA/羊毛角蛋白膜及牙周疾病治疗奠定基础。
