单侧后牙反合患者咬肌中Myf5和MyoD的免疫荧光评估
Immunofluorescence Evaluation of Myf5 and MyoD in Masseter Muscle of Unilateral Posterior Crossbite Patients.
作者信息
Vermiglio Giovanna, Centofanti Antonio, Ramieri Guglielmo, Tepedino Michele, Runci Anastasi Michele, Micali Antonio Girolamo, Arco Alba, Piancino Maria Grazia
机构信息
Department of Biomedical and Dental Science and Morphofunctional Imaging, University of Messina, 98166 Messina, Italy.
Department of Surgical Science, University of Turin, 10124 Torino, Italy.
出版信息
J Funct Morphol Kinesiol. 2020 Nov 7;5(4):80. doi: 10.3390/jfmk5040080.
UNLABELLED
A unilateral posterior crossbite is a malocclusion where the low activity of the affected masseter muscle is compensated by the contralateral muscle hypertrophy. It is still unknown if, in the same condition, myogenesis with new fibre formation takes place.
AIM
the aim of the present study was to evaluate the expression of myogenesis markers, such as Myf5 and MyoD, in masseter muscles of unilateral posterior crossbite patients.
MATERIALS AND METHODS
biopsies from fifteen surgical patients with unilateral posterior crossbites have been analysed by immunofluorescence reactions. The results show the expression of Myf5 and MyoD in the contralateral muscle but not in the ipsilateral one. Moreover, statistical analysis shows the higher number of satellite cells in the contralateral side if compared to the ipsilateral one.
CONCLUSIONS
these results suggest that in contralateral muscle, hyperplastic events take place, as well as hypertrophy.
未标注
单侧后牙反合是一种错牙合畸形，患侧咬肌活动度低，由对侧肌肉肥大代偿。在相同情况下是否会发生伴有新纤维形成的肌生成尚不清楚。
目的
本研究的目的是评估单侧后牙反合患者咬肌中肌生成标志物（如Myf5和MyoD）的表达。
材料与方法
对15例单侧后牙反合手术患者的活检组织进行免疫荧光反应分析。结果显示Myf5和MyoD在对侧肌肉中有表达，而在同侧肌肉中无表达。此外，统计分析表明，与同侧相比，对侧卫星细胞数量更多。
结论
这些结果表明，对侧肌肉中发生了增生性事件以及肥大。
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