Pootong Anek, Norrapong Benja, Cowawintaweewat Suwanna
Southeast Asian J Trop Med Public Health. 2017 Jan;48(1):150-8.
Candida albicans is a common pathogen, especially among immunocompromised patients. It is beginning to show resistance against the azole drug group, which is usually used to treat this pathogen. We studied the antifungal effects of cinnamaldehyde against C. albicans. Germ tube formation of C. albicans exposed to cinnamaldehyde was determined by the crystal violet based method. The effect of cinnamaldehyde on adhesion of C. albicans to buccal epithelial cells was investigated. Proteinase and phospholipase activities of C. albicans in the presence of cinnamaldehyde were assessed using bovine serum albumin agar and egg yolk agar, respectively. In this study, cinnamaldehyde possessed antifungal activity against C. albicans with a minimum inhibitory concentration of 125 μg/ml. At sub-inhibitory concentrations, cinnamaldehyde significantly reduced germ tube formation, proteinase and phospholipase activities in a dose dependent manner (p<0.01). Cinnamaldehyde also significantly inhibited the adhesion of C. albicans to buccal epithelial cells (p<0.01). In our study, cinnamaldehyde had in vitro activity against C. albicans and inhibited some of its virulence factors.
白色念珠菌是一种常见的病原体，在免疫功能低下的患者中尤为常见。它开始对通常用于治疗这种病原体的唑类药物产生耐药性。我们研究了肉桂醛对白色念珠菌的抗真菌作用。通过基于结晶紫的方法测定暴露于肉桂醛的白色念珠菌的芽管形成。研究了肉桂醛对白色念珠菌与颊上皮细胞粘附的影响。分别使用牛血清白蛋白琼脂和蛋黄琼脂评估肉桂醛存在下白色念珠菌的蛋白酶和磷脂酶活性。在本研究中，肉桂醛对白色念珠菌具有抗真菌活性，最低抑菌浓度为125μg/ml。在亚抑菌浓度下，肉桂醛以剂量依赖的方式显著降低芽管形成、蛋白酶和磷脂酶活性（p<0.01）。肉桂醛还显著抑制白色念珠菌与颊上皮细胞的粘附（p<0.01）。在我们的研究中，肉桂醛对白色念珠菌具有体外活性并抑制其一些毒力因子。
