Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207), Marseille, France.
Centre National de Référence-Laboratoire Expert Orthopoxvirus, Institut de Recherche Biomédicale Des Armées (IRBA), 91220, Brétigny-sur-Orge, France.
Sci Rep. 2021 Jan 19;11(1):1798. doi: 10.1038/s41598-021-81376-4.
Success in smallpox eradication was enabled by the absence of non-human reservoir for smallpox virus. However, other poxviruses with a wider host spectrum can infect humans and represent a potential health threat to humans, highlighted by a progressively increasing number of infections by (re)emerging poxviruses, requiring new improved diagnostic and epidemiological tools. We describe here a real-time PCR assay targeting a highly conserved region of the poxvirus genome, thus allowing a pan-Poxvirus detection (Chordopoxvirinae and Entomopoxvirinae). This system is specific (99.8% for vertebrate samples and 99.7% for arthropods samples), sensitive (100% for vertebrate samples and 86.3% for arthropods samples) and presents low limit of detection (< 1000 DNA copies/reaction). In addition, this system could be also valuable for virus discovery and epidemiological projects.
天花的消灭之所以成功，是因为没有非人类的天花病毒储存库。然而，其他宿主范围更广的痘病毒可以感染人类，对人类构成潜在的健康威胁，越来越多的新发和再发痘病毒感染事件凸显了这一问题，这需要新的改进的诊断和流行病学工具。在这里，我们描述了一种针对痘病毒基因组高度保守区域的实时 PCR 检测方法，从而可以实现泛痘病毒检测（Chordopoxvirinae 和 Entomopoxvirinae）。该系统具有特异性（对脊椎动物样本的特异性为 99.8%，对节肢动物样本的特异性为 99.7%）、敏感性（对脊椎动物样本的敏感性为 100%，对节肢动物样本的敏感性为 86.3%），且检测下限低（<1000 个 DNA 拷贝/反应）。此外，该系统对于病毒发现和流行病学项目也具有重要价值。
