Oldal Miklós, Sironen Tarja, Henttonen Heikki, Vapalahti Olli, Madai Mónika, Horváth Győző, Dallos Bianka, Kutas Anna, Földes Fanni, Kemenesi Gábor, Németh Viktória, Bányai Krisztián, Jakab Ferenc
1Virological Research Group, Szentágothai Research Center, University of Pécs, Pécs, Hungary.
2Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
Vector Borne Zoonotic Dis. 2015 May;15(5):317-22. doi: 10.1089/vbz.2014.1731.
As a result of discontinuing vaccination against smallpox after the late 1970s, different orthopoxviruses (OPVs), such as cowpox virus (CPXV), have become a re-emerging healthcare threat among zoonotic pathogens. In Hungary, data on OPV prevalence among its rodent host species have been absent. Here, rodents belonging to four species, i.e., striped field mouse (Apodemus agrarius), yellow-necked mouse (A. flavicollis), wood mouse (A. sylvaticus) and bank vole (Myodes glareolus), were live trapped at 13 sampling plots on a 149-ha area in the Mecsek Mountains, Hungary, from March to September in 2011 and 2012. Rodent sera were collected and screened for OPV-reactive antibodies with an immunfluorescence assay (IFA). Among the 1587 tested rodents, 286 (18.0%) harbored OPV-specific antibodies. Seroprevalence was the highest for the bank vole (71.4%) and the striped field mouse (66.7%). Due to a masting event in the autumn of 2011 across Central Europe, the abundance of bank voles increased drastically in the 2012 season, raising the overall OPV seroprevalence. We provide the first data on OPV occurrence and seroprevalence in rodents in Hungary. The circulation of OPV in rodents in densely populated areas warrants further studies to elucidate the zoonotic potential of OPV in humans.
由于20世纪70年代末后停止了天花疫苗接种，不同的正痘病毒（OPV），如牛痘病毒（CPXV），已成为人畜共患病原体中重新出现的医疗威胁。在匈牙利，缺乏关于其啮齿动物宿主物种中OPV流行情况的数据。在这里，2011年和2012年3月至9月期间，在匈牙利迈切克山脉149公顷区域的13个采样点对属于四个物种的啮齿动物，即条纹田鼠（黑线姬鼠）、黄颈鼠（黄颈姬鼠）、林姬鼠和棕背䶄进行了活体诱捕。收集了啮齿动物血清，并用免疫荧光试验（IFA）筛选OPV反应性抗体。在1587只受试啮齿动物中，286只（18.0%）携带OPV特异性抗体。血清阳性率在棕背䶄（71.4%）和条纹田鼠（66.7%）中最高。由于2011年秋季中欧各地出现种子大年现象，2012年季节棕背䶄的数量急剧增加，提高了总体OPV血清阳性率。我们提供了匈牙利啮齿动物中OPV发生情况和血清阳性率的首批数据。OPV在人口密集地区的啮齿动物中的传播值得进一步研究，以阐明OPV对人类的人畜共患病潜力。
