Department of Orthopedic Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, People's Republic of China.
Int J Nanomedicine. 2021 Jan 14;16:371-381. doi: 10.2147/IJN.S287875. eCollection 2021.
Efficient approaches to reliably improving wound healing in diabetic patients remain to be developed. Exosomes are nanomaterials from which therapeutically active microRNAs (miRNAs) can be isolated. In the present report, we therefore isolated circulating exosome-derived miRNAs from patients with diabetes and assessed the impact of these molecules on wound healing.
Exosomes were isolated from the serum of control or diabetic patients (Con-Exos and Dia-Exos, respectively), after which the effects of these exosomes on cellular activity and wound healing were assessed.
We determined that miR-20b-5p was overexpressed in Dia-Exos and that it functioned by impairing wound repair by suppressing vascular endothelial growth factor A (VEGFA) expression. Consistent with such a model, the administration of Dia-Exos or this miRNA both in vivo and in vitro was sufficient to slow wound repair.
Dia-Exos exhibit significant increases in miR-20b-5p relative to Con-Exos, and this miRNA can be transferred into HSFs wherein it can suppress VEGFA expression and thereby slow the process of wound healing.
开发可靠地改善糖尿病患者伤口愈合的有效方法仍然是必要的。外泌体是一种纳米材料，可以从中分离出具有治疗作用的微小 RNA（miRNA）。在本报告中，我们从糖尿病患者的血清中分离出循环外泌体衍生的 miRNA，并评估这些分子对伤口愈合的影响。
分别从对照组或糖尿病患者（Con-Exos 和 Dia-Exos）的血清中分离出外泌体，然后评估这些外泌体对细胞活性和伤口愈合的影响。
我们确定 miR-20b-5p 在 Dia-Exos 中过度表达，它通过抑制血管内皮生长因子 A（VEGFA）的表达来损害伤口修复。与这种模型一致，体内和体外给予 Dia-Exos 或这种 miRNA 足以减缓伤口修复。
与 Con-Exos 相比，Dia-Exos 表现出 miR-20b-5p 的显著增加，并且这种 miRNA 可以被转移到 HSFs 中，在其中它可以抑制 VEGFA 的表达，从而减缓伤口愈合过程。
