Department of Microbiology, All India Institute of Medical Sciences (AIIMS), New Delhi, Delhi, 110029, India.
Department of Microbiology, University College of Medical Sciences (UCMS) & Guru TegBahadur (GTB) Hospital, New Delhi, Delhi, India.
Mycopathologia. 2021 May;186(2):199-211. doi: 10.1007/s11046-020-00500-6. Epub 2021 Jan 20.
Cryptococcosis is a life-threatening infection caused by Cryptococcus neoformans and C. gattii species complex. In the present study, to understand the molecular epidemiology of 208 clinical isolates of Cryptococcus from different parts of India, multilocus sequence typing (MLST) using ISHAM MLST consensus scheme for C. neoformans/C. gattii species complex was used. MLST analysis yielded a total of 10 Sequence Types (STs)-7 STs for C. neoformans and 3 for C. gattii species complex. The majority of isolates identified as C. neoformans belonged to molecular type VNI with predominant STs 31 and 93. Only 3 isolates of C. gattii species complex were obtained, belonging to ST58 and ST215 of VGI and ST69 of VGIV. Phylogenetic analysis revealed less diversity among the clinical Indian isolates compared to the global MLST database. No association between prevalent STs and HIV status, geographical origin or minimum inhibitory concentration (MIC) could be established.
隐球菌病是由新型隐球菌和格特隐球菌复合群引起的危及生命的感染。在本研究中，为了了解来自印度不同地区的 208 株隐球菌临床分离株的分子流行病学，使用隐球菌新型隐球菌/格特隐球菌复合群的 ISHAM MLST 共识方案进行了多位点序列分型（MLST）。MLST 分析共产生了 10 种序列型（ST）-7 种新型隐球菌 ST 和 3 种格特隐球菌复合群 ST。大多数鉴定为新型隐球菌的分离株属于分子型 VNI，主要 ST 为 31 和 93。仅获得了 3 株格特隐球菌复合群的分离株，属于 VGI 的 ST58 和 ST215 以及 VGIV 的 ST69。系统发育分析显示，与全球 MLST 数据库相比，印度临床分离株的多样性较低。未能确定流行 ST 与 HIV 状态、地理位置或最低抑菌浓度（MIC）之间的关联。
