Aspenberg P, Albrektsson T, Lohmander L S, Thorngren K G
Department of Orthopaedics, University of Lund, Sweden.
J Biomed Eng. 1988 Jan;10(1):70-3. doi: 10.1016/0141-5425(88)90029-5.
A titanium implant in which a conduit is gradually filled with ingrowing bone (the Bone Harvest Chamber) has been modified to allow continuous local treatment of the conduit tissue with biochemical agents. Implants were inserted bilaterally in rabbit tibiae. The tissue content of the bone ingrowth conduits was studied with histology, 99mTc-MDP scintimetry and measurements of total calcium content. Bone was formed in the conduit by endochondral formation starting at both ends and continuing until fusion in the middle. After 2 weeks the bone had not yet met in the middle where fibrous tissue was seen. In eight animals 3H-proline was applied via one of the chambers, with the contralateral chamber as a saline-treated control. The collagen of the harvested tissue from the 3H-proline treated side had a 3H-hydroxyproline content 1000 times greater than had the control side. The 'drug test chamber' makes possible the study of local effects of drugs on healing of mature bone in vivo.
一种带有逐渐被内生骨填充的管道（骨采集腔）的钛植入物已被改进，以允许用生化制剂对管道组织进行持续局部治疗。将植入物双侧插入兔胫骨。通过组织学、99mTc-MDP闪烁扫描法和总钙含量测量研究了骨生长管道的组织成分。骨通过软骨内成骨在管道两端开始形成，并持续到中间融合。2周后，中间的骨尚未会合，可见纤维组织。在8只动物中，通过其中一个腔室应用3H-脯氨酸，对侧腔室作为生理盐水处理的对照。来自3H-脯氨酸处理侧的采集组织的胶原蛋白的3H-羟脯氨酸含量比对照侧高1000倍。“药物测试腔”使得研究药物对体内成熟骨愈合的局部作用成为可能。
