Kim Young-Kee, Kwak Myoung Hai, Hong Ja-Ram, Kim Hoe-Won, Jo Sangjin, Sohn Jung-Yeon, Cheon Se-Hwan, Kim Ki-Joong
Division of Life Sciences, Korea University, Seoul, Korea.
Department of Plant Resources, National Institute of Biological Resources, Incheon, Korea.
Mitochondrial DNA B Resour. 2017 Oct 14;2(2):704-706. doi: 10.1080/23802359.2017.1390410.
In this study, we determined the complete chloroplast sequence of (Thunb.) Spreng. (Orchidaceae) (NCBI acc. no. KX871237), an endangered plant species protected by the national law of Korea. The gene order and gene content of the plastome are similar to those of typical angiosperm plastomes. The 11 genes, which are usually lost in orchid plastomes, are intact in the plastome. The complete plastome is 155,353 bp in length and consists of a large single copy of 84,833 bp and a small single copy of 17,718 bp, separated by two inverted repeats of 26,401 bp. The plastome contains 113 genes, of which 79 are protein-coding genes, 30 are tRNA genes, and four are rRNA genes. Sixteen genes contain one intron and two genes (P, 3) have two introns. A total of 76 simple sequence repeat (SSR) loci, which consist of 58 mono-SSR, 17 di-SSR, and 1 tri-SSR, are scattered along the plastome. Some of these plastome SSR and high sequence divergent regions may be useful for development of genetic markers for the populations of and other congeneric species. Phylogenetic analysis identified the sister relationship between and within the tribe Orchideae.
在本研究中，我们测定了受韩国国家法律保护的濒危植物物种（兰科）（NCBI登录号：KX871237）的完整叶绿体序列。该植物叶绿体基因组的基因顺序和基因内容与典型被子植物叶绿体基因组相似。通常在兰花叶绿体基因组中缺失的11个基因在该植物叶绿体基因组中是完整的。完整的叶绿体基因组长度为155,353 bp，由一个84,833 bp的大单拷贝和一个17,718 bp的小单拷贝组成，两者由两个26,401 bp的反向重复序列隔开。叶绿体基因组包含113个基因，其中79个是蛋白质编码基因，30个是tRNA基因，4个是rRNA基因。16个基因含有一个内含子，两个基因（P、3）有两个内含子。沿着该植物叶绿体基因组共散布着76个简单序列重复（SSR）位点，其中包括58个单核苷酸SSR、17个双核苷酸SSR和1个三核苷酸SSR。这些叶绿体基因组SSR和高序列分歧区域中的一些可能有助于开发该植物及其他同属物种种群的遗传标记。系统发育分析确定了该植物与兰科部落内的之间的姐妹关系。
