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濒危兰花(兰科)的完整质体基因组序列

The complete plastome sequence of the endangered orchid (Orchidaceae).

作者信息

Kim Young-Kee, Kwak Myoung Hai, Chung Myong Gi, Kim Hoe-Won, Jo Sangjin, Sohn Jung-Yeon, Cheon Se-Hwan, Kim Ki-Joong

机构信息

Division of Life Sciences, Korea University, Seoul, Korea.

Department of Plant Resources, National Institute of Biological Resources, Incheon, Korea.

出版信息

Mitochondrial DNA B Resour. 2017 Oct 18;2(2):725-727. doi: 10.1080/23802359.2017.1390411.

DOI:10.1080/23802359.2017.1390411
PMID:33473960
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7799478/
Abstract

In this study, we determined the complete chloroplast sequence of Lindl. (Orchidaceae) (NCBI acc. no. KY354040), an endangered plant species protected by the national law of Korea. The gene order and number in the plastome were similar to a typical Orchid plastome. The complete plastome was 149,859 bp in length and consisted of a large single copy region of 85,187 bp and a small single copy region of 13,766 bp; these were separated by two inverted repeats of 25,453 bp. The plastome contained 103 genes of which 69 were protein-coding genes, 30 were tRNA genes and four were rRNA genes. Fourteen genes contained one intron and two genes (P, and 3) had two introns. The AT content of the plastome was 60.0% and a total of 62 simple sequence repeat regions were identified in the plastome. Phylogenetic analysis also identified as a closely related sister to , suggesting that the hemisaprophytic nature of is derived recently from a common leafy ancestor.

摘要

在本研究中,我们测定了韩国国家法律保护的濒危植物物种Lindl.(兰科)(NCBI登录号KY354040)的完整叶绿体序列。质体基因组中的基因顺序和数量与典型的兰花质体基因组相似。完整的质体基因组长度为149,859 bp,由一个85,187 bp的大单拷贝区域和一个13,766 bp的小单拷贝区域组成;它们被两个25,453 bp的反向重复序列隔开。质体基因组包含103个基因,其中69个是蛋白质编码基因,30个是tRNA基因,4个是rRNA基因。14个基因含有一个内含子,两个基因(P和3)有两个内含子。质体基因组的AT含量为60.0%,质体基因组中总共鉴定出62个简单序列重复区域。系统发育分析还确定 为 的近缘姐妹,这表明 的半腐生性质最近源自一个常见的多叶祖先。

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