Jo Sangjin, Kim Hoe-Won, Kim Young-Kee, Cheon Se-Hwan, Kim Ki-Joong
Division of Life Sciences, Korea University, Seoul, Korea.
Mitochondrial DNA B Resour. 2017 Jan 16;2(1):26-28. doi: 10.1080/23802359.2016.1233468.
In this study, we determined the complete plastome sequence of (Eckl.) A. DC. (Apocynaceae) (NCBI acc. no. KX364402). The gene order and structure of the plastome are similar to those of a typical angiosperm. The complete plastome is 155,297 bp in length, and consists of a large single-copy region of 85,586 bp and a small single-copy region of 18,131 bp, which are separated by two inverted repeats of 25,792 bp. The plastome contains 113 genes, of which 79 are protein-coding genes, 30 are tRNA genes and 4 are rRNA genes. Sixteen genes contained one intron and two genes have two introns. The average A-T content of the plastome is 62.0%. A total of 31 simple sequence repeat loci were identified within the genome. Phylogenetic analysis revealed that is a member of the paraphyletic subfamily Rauvolfioideae of Apocynaceae. The sister group relationship of to the Apocynoideae-Asclepiadoideae clade is supported by 100% bootstrap values.
在本研究中,我们测定了(Eckl.)A. DC.(夹竹桃科)的完整质体基因组序列(NCBI登录号:KX364402)。该质体基因组的基因顺序和结构与典型被子植物的相似。完整的质体基因组长度为155,297 bp,由一个85,586 bp的大单拷贝区域和一个18,131 bp的小单拷贝区域组成,这两个区域被两个25,792 bp的反向重复序列隔开。质体基因组包含113个基因,其中79个是蛋白质编码基因,30个是tRNA基因,4个是rRNA基因。16个基因含有一个内含子,两个基因有两个内含子。质体基因组的平均A-T含量为62.0%。在基因组内共鉴定出31个简单序列重复位点。系统发育分析表明,是夹竹桃科副系罗布麻亚科的一个成员。与夹竹桃亚科-萝摩亚科分支的姐妹群关系得到了100%的自展支持值。