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濒危兰花(兰科)的完整质体基因组序列

The complete plastome sequence of the endangered orchid (Orchidaceae).

作者信息

Kim Young-Kee, Kwak Myoung Hai, Hong Ja-Ram, Kim Hoe-Won, Jo Sangjin, Sohn Jung-Yeon, Cheon Se-Hwan, Kim Ki-Joong

机构信息

Division of Life Sciences, Korea University, Seoul, Korea.

Department of Plant Resources, National Institute of Biological Resources, Incheon, Korea.

出版信息

Mitochondrial DNA B Resour. 2017 Oct 14;2(2):701-703. doi: 10.1080/23802359.2017.1390408.

Abstract

In this study, we report the complete plastome sequence of (F.Maek.) Ormerod (Orchidaceae) (NCBI acc. no. KY354041), an endangered plant species protected by the national law of Korea. The gene order and number in the plastome were similar to a typical orchid plastome. The complete plastome was 147,614 bp in length and consisted of a large single copy region of 81,617 bp and a small single copy region of 13,673 bp, separated by two inverted repeats of 26,162 bp. The plastome contained 103 genes, of which 69 were protein-coding genes, 30 were tRNA genes, and four were rRNA genes. Fourteen genes contained one intron and two genes (P and 3) had two introns. The AT content of the plastome was 60.5%. A total of 74 simple sequence repeat regions were identified from the plastome. Phylogenetic analysis determined that was a member of the tribe Cranichideae and revealed the sister group relationship between and within the tribe Cranichideae.

摘要

在本研究中,我们报告了受韩国国家法律保护的濒危植物物种(F.Maek.)奥默罗德(兰科)(NCBI登录号KY354041)的完整质体基因组序列。该质体基因组中的基因顺序和数量与典型的兰花质体基因组相似。完整的质体基因组长度为147,614 bp,由一个81,617 bp的大单拷贝区域和一个13,673 bp的小单拷贝区域组成,这两个区域被两个26,162 bp的反向重复序列隔开。质体基因组包含103个基因,其中69个是蛋白质编码基因,30个是tRNA基因,4个是rRNA基因。14个基因含有一个内含子,两个基因(P和3)有两个内含子。质体基因组的AT含量为60.5%。从质体基因组中总共鉴定出74个简单序列重复区域。系统发育分析确定该物种是克兰尼科族的成员,并揭示了该物种与克兰尼科族内的另一个物种之间的姐妹群关系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a6/7799528/1cf919a484df/TMDN_A_1390408_F0001_C.jpg

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