Chen Zi-Shu, Xu Zhi, Ma Li, Wang Xing
College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China.
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, China.
Mitochondrial DNA B Resour. 2018 Jan 5;3(1):78-80. doi: 10.1080/23802359.2017.1422406.
In this study, the complete mitochondrial genome (mitogenome) of was sequenced for the first time by traditional PCR amplification and primer walking methods. As a circular DNA molecule, the entire mitogenome is 15,532 bp in length (GeneBank accession no. KT362742), and consists of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and an AT-rich region. The nucleotide composition is A (39.9%), C (12.0%), G (7.6%), and T (40.4%). Based onthe amino acid sequences of 13 PCGs from 43 noctuoid species as ingroups and two drepanoid species as outgroups, the phylogenetic trees was constructed. The phylogenetic relationships of the families among Noctuoidea are (Notodontidae, (Erebidae, (Nolidae, Noctuidae)). However, the phylogenetic position of is still unclear in the family Erebidae that is a monophyletic clade strongly supported by the bootstrap value of 100%.
在本研究中，首次通过传统PCR扩增和引物步移法对[物种名称缺失]的完整线粒体基因组（线粒体基因组）进行了测序。作为一个环状DNA分子，整个线粒体基因组长度为15,532 bp（基因库登录号KT362742），由13个蛋白质编码基因（PCG）、22个转移RNA（tRNA）基因、两个核糖体RNA（rRNA）基因和一个富含AT的区域组成。核苷酸组成为A（39.9%）、C（12.0%）、G（7.6%）和T（40.4%）。基于43种夜蛾类物种的13个PCG的氨基酸序列作为内群，以及两种尺蛾类物种作为外群，构建了系统发育树。夜蛾总科中各科的系统发育关系为（舟蛾科，（灯蛾科，（毒蛾科，夜蛾科）））。然而，在灯蛾科中[物种名称缺失]的系统发育位置仍不清楚，灯蛾科是一个单系类群，自展值为100%，得到了有力支持。
