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下一代测序技术获得了圈养林麝（反刍亚目：麝科）的完整线粒体基因组。

Next generation sequencing yields the complete mitogenome of captive forest musk deer, (Ruminantia: Moschidae).

作者信息

Yang Chao, Tang Jie, Bian Kun, Suo Li-Juan, Yuan Hao, Wang Yan, Huang Yuan

机构信息

Shaanxi Institute of Zoology, Xi'an, China.

School of Life Sciences, Shaanxi Normal University, Xi'an, China.

出版信息

Mitochondrial DNA B Resour. 2018 Apr 23;3(2):472-473. doi: 10.1080/23802359.2018.1462670.

DOI:10.1080/23802359.2018.1462670
PMID:33474208
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7800556/
Abstract

is an endangered species, but its captive populations are valuable on musk secretions in traditional Chinese medicine and perfume manufacture. The mitogenome of was 16,353 bp in size. Stop codons in 13 PCGs were all typical types except incomplete stop codon T for , and , and TA for No tandem repeat was found in control region. Phylogenetic analysis indicated that Moschidae has the closest relationship with Bovidae. We supported that should be categorized into two subspecies, and suggested that the status of JQ608470 should be further investigated.

摘要

是一种濒危物种，但其圈养种群在传统中药和香水制造中的麝香分泌方面具有重要价值。该物种的线粒体基因组大小为16353bp。13个蛋白质编码基因中的终止密码子均为典型类型，除了 、 和 的不完全终止密码子T以及 的TA。控制区未发现串联重复序列。系统发育分析表明麝科与牛科关系最为密切。我们支持应将该物种分为两个亚种，并建议对JQ608470的地位进行进一步调查。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81fb/7800556/0625ce8dac67/TMDN_A_1462670_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81fb/7800556/0625ce8dac67/TMDN_A_1462670_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81fb/7800556/0625ce8dac67/TMDN_A_1462670_F0001_B.jpg

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本文引用的文献

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Mitochondrial DNA B Resour. 2017 Nov 25;2(2):860-861. doi: 10.1080/23802359.2017.1407715.
2
Species Delimitation in the Genus Moschus (Ruminantia: Moschidae) and Its High-Plateau Origin.麝属（反刍亚目：麝科）的物种界定及其高原起源
PLoS One. 2015 Aug 17;10(8):e0134183. doi: 10.1371/journal.pone.0134183. eCollection 2015.
3
Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads--a baiting and iterative mapping approach.
直接从基因组下一代测序读段重建线粒体基因组——一种诱饵和迭代映射方法。
Nucleic Acids Res. 2013 Jul;41(13):e129. doi: 10.1093/nar/gkt371. Epub 2013 May 9.
4
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Mitochondrial DNA. 2013 Oct;24(5):501-3. doi: 10.3109/19401736.2013.770504. Epub 2013 Apr 11.
5
MITOS: improved de novo metazoan mitochondrial genome annotation.MITOS：改进的从头后生动物线粒体基因组注释。
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MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space.MrBayes 3.2：在大型模型空间中进行高效的贝叶斯系统发育推断和模型选择。
Syst Biol. 2012 May;61(3):539-42. doi: 10.1093/sysbio/sys029. Epub 2012 Feb 22.
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Partitionfinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses.Partitionfinder：用于系统发育分析的分区方案和替代模型的联合选择。
Mol Biol Evol. 2012 Jun;29(6):1695-701. doi: 10.1093/molbev/mss020. Epub 2012 Jan 20.
8
Genetic diversity of captive forest musk deer (Moschus berezovskii) inferred from the mitochondrial DNA control region.基于线粒体DNA控制区推断圈养林麝（Moschus berezovskii）的遗传多样性
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Bioinformatics. 2006 Nov 1;22(21):2688-90. doi: 10.1093/bioinformatics/btl446. Epub 2006 Aug 23.
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Molecular and morphological phylogenies of ruminantia and the alternative position of the moschidae.反刍亚目动物的分子与形态系统发育以及麝科的另类位置
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