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var. 的首个完整叶绿体基因组,对毛茛科系统发育的意义。

The first complete chloroplast genome of var. , with implication for the phylogeny of Ranunculaceae.

作者信息

Mamut Reyim, Yisilam Gulbar, Zhang Hongwei, Hina Faiza, Yang Zhaoping

机构信息

College of Life Sciences and Technology, Xinjiang University, Urumqi, China.

Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou, China.

出版信息

Mitochondrial DNA B Resour. 2018 Aug 17;3(2):951-952. doi: 10.1080/23802359.2018.1501308.

DOI:10.1080/23802359.2018.1501308
PMID:33474376
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7800659/
Abstract

is one of the most important medicinal plant genera in eastern Asia. To better understand the evolution of this genus, the complete chloroplast genome of var. was obtained by next-generation sequencing. The plastome of var. is 155,426 bp in length, and consists of large (LSC, 84,488 bp) and small (SSC, 17,402bp) single-copy regions, separated by pair of inverted repeat regions (IRs, 26,768 bp). It harbours 111 unique genes, including 78 protein-coding genes, 29 transfer RNA genes, and four ribosomal RNA genes. and were pseudogenized due to incomplete duplication in IR regions. The nucleotide composition is asymmetric (30.5% A, 19.4% C, 18.7% G, and 31.3% T) with an overall G + C content of 38.2%. The phylogeny of Ranunculaceae based on 75 CDSs of 27 taxa showed that Ranunculoideae is paraphyletic and thus needs redefinition.

摘要

是东亚最重要的药用植物属之一。为了更好地理解该属的进化,通过二代测序获得了[物种名称]变种的完整叶绿体基因组。[物种名称]变种的质体基因组长度为155,426 bp,由大单拷贝区(LSC,84,488 bp)和小单拷贝区(SSC,17,402 bp)组成,二者由一对反向重复区(IRs,26,768 bp)隔开。它含有111个独特基因,包括78个蛋白质编码基因、29个转运RNA基因和4个核糖体RNA基因。[具体基因名称1]和[具体基因名称2]由于在IR区域的不完全重复而成为假基因。核苷酸组成不对称(A占30.5%,C占19.4%,G占18.7%,T占31.3%),总体G + C含量为38.2%。基于27个分类群的75个编码序列构建的毛茛科系统发育树表明,毛茛亚科是并系的,因此需要重新定义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b92/7800659/9c95f606af24/TMDN_A_1501308_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b92/7800659/9c95f606af24/TMDN_A_1501308_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b92/7800659/9c95f606af24/TMDN_A_1501308_F0001_C.jpg

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本文引用的文献

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NOVOPlasty: de novo assembly of organelle genomes from whole genome data.NOVOPlasty:从头组装细胞器基因组的全基因组数据。
Nucleic Acids Res. 2017 Feb 28;45(4):e18. doi: 10.1093/nar/gkw955.
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RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies.RAxML 版本 8:用于系统发育分析和大型系统发育后分析的工具。
Bioinformatics. 2014 May 1;30(9):1312-3. doi: 10.1093/bioinformatics/btu033. Epub 2014 Jan 21.
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[Population characteristics of Coptis chinensis var. brevisepala in Anhui Province and its endangering mechanism].[安徽省短萼黄连的种群特征及其致危机制]
Ying Yong Sheng Tai Xue Bao. 2005 Aug;16(8):1394-8.
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MrBayes 3: Bayesian phylogenetic inference under mixed models.MrBayes 3:混合模型下的贝叶斯系统发育推断。
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