Zhang Yunyan, Dong Jiaxian, Feng Yu, Wang Zhongsheng, Li Pan
Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education College of Life Sciences, Zhejiang University, Hangzhou, China.
College of Life Sciences, Nanjing University, Nanjing, China.
Mitochondrial DNA B Resour. 2018 Mar 14;3(1):370-372. doi: 10.1080/23802359.2018.1450684.
, serving as a valuable medicinal plant, is a critically endangered and national key protected species of China. In the past, researches regarding this species mainly focused on its medicinal ingredients, whereas little effort has been made on its genetic information. Here we determined the first complete chloroplast genome of using genome skimming approach. The cp genome was 154,549 bp long, with a large single-copy region (LSC) of 84,409 bp and a small single-copy region (SSC) of 17,256 bp separated by a pair of inverted repeats (IRs) of 26,442 bp. It encodes 113 unique genes, including 79 protein-coding genes, 30 transfer RNA genes, and four ribosomal RNA genes. Besides, we reconstructed the phylogeny of Ranunculaceae based on the previously reported cp genomes of related taxa. A maximum likelihood (ML) phylogenetic analysis strongly supported the monophyly of Hydrastidoideae, Coptidoideae, and Thalictroideae, while Ranunculoideae was found to be paraphyletic.
作为一种珍贵的药用植物，是中国国家重点保护的极度濒危物种。过去，关于该物种的研究主要集中在其药用成分上，而对其遗传信息的研究较少。在此，我们采用基因组浅层测序方法首次测定了其完整的叶绿体基因组。叶绿体基因组长度为154,549 bp，有一个84,409 bp的大单拷贝区域（LSC）和一个17,256 bp的小单拷贝区域（SSC），由一对26,442 bp的反向重复序列（IR）隔开。它编码113个独特基因，包括79个蛋白质编码基因、30个转运RNA基因和4个核糖体RNA基因。此外，我们基于先前报道的相关类群的叶绿体基因组重建了毛茛科的系统发育。最大似然（ML）系统发育分析强烈支持黄连亚科、唐松草亚科和铁筷子亚科的单系性，而毛茛亚科被发现是并系的。
