Fernando Lourds M, Golliday Kyrionna, Boateng Ruby, Allen Anna K
Department of Biology, Howard University, Washington DC USA.
The Jackson Laboratory, Bar Harbor, ME USA.
MicroPubl Biol. 2021 Jan 14;2021. doi: 10.17912/micropub.biology.000353.
We have generated a WEE-1.3 strain in wherewe have endogenously tagged the C-terminus with GFP In this publication we demonstrate that this new strain exhibits the same expression localization pattern as the WEE-1.3 antibody and N-terminally endogenously GFP-tagged WEE-1.3 strain that have been previously published. We also show for the first time that endogenously tagging WEE-1.3 at either termini does not affect the reproductive function of the worms.
我们构建了一个WEE-1.3菌株，在该菌株中我们用绿色荧光蛋白（GFP）对其C末端进行了内源性标记。在本出版物中，我们证明这个新菌株表现出与先前发表的WEE-1.3抗体以及N末端内源性GFP标记的WEE-1.3菌株相同的表达定位模式。我们还首次表明，在任何一个末端对内源性WEE-1.3进行标记都不会影响线虫的生殖功能。
