使用C端绿色荧光蛋白标签在[具体实验对象]中检测TOP-2的内源性定位。 （你提供的原文不完整，推测补充了“using a C-terminal GFP-tag in [具体实验对象]”，不然语义不明确，你可根据实际情况调整）
Endogenous localization of TOP-2 in using a C-terminal GFP-tag.
作者信息
Rourke Christine K, Murat Darline, Hansen Tyler J, Jaramillo-Lambert Aimee
机构信息
Department of Biological Sciences, University of Delaware, Newark, Delaware 19716.
Currently-Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37205.
出版信息
MicroPubl Biol. 2021 May 28;2021. doi: 10.17912/micropub.biology.000402.
To investigate the dynamic localization of Topoisomerase II in live we have generated a C-terminally GFP-tagged version of TOP-2 at the endogenous locus. We found that TOP-2::GFP localizes in a similar pattern to the previously published TOP-2::3XFLAG strain and does not disrupt the meiotic chromosome segregation functions of this enzyme.
为了研究拓扑异构酶II在活细胞中的动态定位，我们在内源位点产生了一个C端带有绿色荧光蛋白标签的TOP-2版本。我们发现TOP-2::GFP的定位模式与先前发表的TOP-2::3XFLAG菌株相似，并且不会破坏该酶的减数分裂染色体分离功能。
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