Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, UK.
Department of Clinical Medicine, The MR Research Center, Aarhus University, Aarhus, Denmark.
Methods Mol Biol. 2021;2216:473-480. doi: 10.1007/978-1-0716-0978-1_28.
Sodium handling is a key physiological hallmark of renal function. Alterations are generally considered a pathophysiologic event associated with kidney injury, with disturbances in the corticomedullary sodium gradient being indicative of a number of conditions. This experimental protocol review describes the individual steps needed to perform Na MRI; allowing accurate monitoring of the renal sodium distribution in a step-by-step experimental protocol for rodents.This chapter is based upon work from the PARENCHIMA COST Action, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers. This experimental protocol chapter is complemented by two separate chapters describing the basic concept and data analysis.
钠处理是肾功能的一个关键生理标志。改变通常被认为是与肾脏损伤相关的病理生理事件，皮质髓质钠梯度的紊乱表明存在多种情况。本实验方案综述描述了进行 Na MRI 所需的各个步骤；允许在啮齿动物的逐步实验方案中准确监测肾脏钠的分布。这一章基于 PARENCHIMA COST 行动的工作，该行动是一个由欧洲科学技术合作组织（COST）欧盟计划资助的社区驱动网络，旨在提高肾脏 MRI 生物标志物的重现性和标准化。本实验方案章节由另外两个描述基本概念和数据分析的章节补充。
