Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Association, Berlin, Germany.
Siemens Healthcare, Berlin, Germany.
Methods Mol Biol. 2021;2216:403-417. doi: 10.1007/978-1-0716-0978-1_23.
Renal hypoxia is generally accepted as a key pathophysiologic event in acute kidney injury of various origins, and has also been suggested to play a role in the development of chronic kidney disease. Here we describe a step-by-step experimental protocol for indirect monitoring of renal blood oxygenation in rodents via the deoxyhemoglobin sensitive MR parameters T* and T-a contrast mechanism known as the blood oxygenation level dependent (BOLD) effect. Since an absolute quantification of renal oxygenation from T*/T remains challenging, the effects of controlled and standardized variations in the fraction of inspired oxygen are used for bench marking. This MRI method may be useful for investigating renal blood oxygenation of small rodents in vivo under various experimental (patho)physiological conditions.This chapter is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers. This experimental protocol chapter is complemented by two separate chapters describing the basic concept and data analysis.
肾缺氧被普遍认为是各种来源的急性肾损伤的关键病理生理事件，也被认为在慢性肾脏病的发展中起作用。在这里，我们描述了一个通过脱氧血红蛋白敏感的磁共振参数 T和 T-a 对比机制（即血氧水平依赖（BOLD）效应）间接监测啮齿动物肾血氧的逐步实验方案。由于从 T/T 绝对定量肾氧仍然具有挑战性，因此使用受控和标准化的吸入氧分数变化来进行基准测试。这种 MRI 方法可用于在各种实验（病理）生理条件下研究小啮齿动物的肾血供。
这一章是基于 COST 行动 PARENCHIMA 的工作，这是一个由欧洲合作科学技术（COST）计划资助的社区驱动网络，旨在提高肾脏 MRI 生物标志物的可重复性和标准化。这个实验方案章节由另外两个描述基本概念和数据分析的章节补充。
