Working Group Integrative Kidney Physiology, Institute of Physiology, Charité - University Medicine Berlin, Berlin, Germany.
Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Free University Berlin, Berlin, Germany.
Methods Mol Biol. 2021;2216:327-347. doi: 10.1007/978-1-0716-0978-1_19.
Renal tissue hypoperfusion and hypoxia are early key elements in the pathophysiology of acute kidney injury of various origins, and may also promote progression from acute injury to chronic kidney disease. Here we describe methods to study control of renal hemodynamics and tissue oxygenation by means of invasive probes in anesthetized rats. Step-by-step protocols are provided for two setups, one for experiments in laboratories for integrative physiology and the other for experiments within small-animal magnetic resonance scanners.This publication is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers. This experimental protocol chapter is complemented by a separate chapter describing the basic concepts of quantitatively assessing renal perfusion and oxygenation with invasive probes.
肾脏组织低灌注和缺氧是各种来源的急性肾损伤病理生理学的早期关键因素，也可能促进急性损伤向慢性肾脏病的进展。在这里，我们描述了在麻醉大鼠中使用侵入性探针研究肾脏血液动力学和组织氧合控制的方法。为两种设置提供了逐步方案，一种用于综合生理学实验室的实验，另一种用于小型动物磁共振扫描仪内的实验。本出版物基于成本行动 PARENCHIMA 的工作，这是一个由欧洲科学技术合作组织（COST）计划资助的社区驱动网络，旨在提高肾脏 MRI 生物标志物的可重复性和标准化。该实验方案章节由另一个单独的章节补充，该章节描述了使用侵入性探针定量评估肾脏灌注和氧合的基本概念。
