Šefcová Miroslava Anna, Larrea-Álvarez Marco, Larrea-Álvarez César Marcelo, Karaffová Viera, Ortega-Paredes David, Vinueza-Burgos Christian, Ševčíková Zuzana, Levkut Mikuláš, Herich Róbert, Revajová Viera
School of Biological Sciences and Engineering, Yachay-Tech University Hacienda San José, Urcuquí-Imbabura 100650, Ecuador.
Research Unit, Life Science Initiative (LSI), Quito 170102, Ecuador.
Animals (Basel). 2021 Jan 19;11(1):235. doi: 10.3390/ani11010235.
This research was conducted to investigate if the administration of the probiotic could influence body weight, intestinal morphometry and the cecal cytokine response in -infected chickens. Seventy-two 1-day old COBB 500 male chicks were allocated randomly into four experimental groups. (I) Control group (C), in which chicks were left untreated. (II) LB group, treated with . (III) Cj group, infected with and (IV) coexposure group in which both bacteria were administered. Body weight was registered and then all birds were slaughtered; samples from the small intestine and caecum were collected at 4- and 7-days post infection. The experiment lasted eleven days. Villi height and crypt depth ratios of the duodenum, jejunum and ileum were evaluated using appropriate software, while reverse transcription quantitative PCR (RT-qPCR) was utilized for assessing transcript levels of key cecal inflammatory cytokines (IL-1β, IL-18, IL-17, IL-15, IL13 and IL-4). -infected birds showed lower body weight values than those supplemented with the probiotic; these birds, in turn, proved to be heavier than those reared under control conditions. administration improved morphometrical parameters of the duodenum, jejunum and ileum; in general, villi were larger and crypts deeper than those identified in control conditions. Moreover, the negative effects elicited by were not observed in chickens exposed to the probiotic. Significant differences were also determined with regards to transcript abundance of all evaluated cytokines in the caecum. induced a downregulation of the studied interleukins; however, such a response was heightened by administration of , with an increase rate of transcription that promoted a more effective response to a infection. The effects of experimental treatments proved to vary between sampling points. Conclusively, these results demonstrate that lessens the negative effects elicited by on body weight by alleviating the impact on intestinal morphometry and cecal cytokine response, which ultimately improve chicken growth performance.
本研究旨在调查给予益生菌是否会影响感染鸡的体重、肠道形态以及盲肠细胞因子反应。72只1日龄的科宝500雄性雏鸡被随机分为四个实验组。（I）对照组（C），雏鸡不进行处理。（II）LB组，用……处理。（III）Cj组，感染……和（IV）共暴露组，同时给予两种细菌。记录体重，然后宰杀所有鸡；在感染后4天和7天收集小肠和盲肠样本。实验持续11天。使用适当软件评估十二指肠、空肠和回肠的绒毛高度与隐窝深度比值，同时利用逆转录定量PCR（RT-qPCR）评估关键盲肠炎性细胞因子（IL-1β、IL-18、IL-17、IL-15、IL-13和IL-4）的转录水平。感染……的鸡体重值低于补充益生菌的鸡；反过来，这些鸡比在对照条件下饲养的鸡更重。给予……改善了十二指肠、空肠和回肠的形态学参数；总体而言，绒毛比对照条件下更大，隐窝更深。此外，在暴露于益生菌的鸡中未观察到……引起的负面影响。在盲肠中所有评估细胞因子的转录丰度方面也确定了显著差异。……诱导所研究的白细胞介素下调；然而，给予……会增强这种反应，转录增加率促进了对……感染更有效的反应。实验处理的效果在不同采样点之间有所不同。总之，这些结果表明，……通过减轻对肠道形态和盲肠细胞因子反应的影响，减轻了……对体重的负面影响，最终改善了鸡的生长性能。
