Šefcová Miroslava, Larrea-Álvarez Marco, Larrea-Álvarez César, Revajová Viera, Karaffová Viera, Koščová Jana, Nemcová Radomíra, Ortega-Paredes David, Vinueza-Burgos Christian, Levkut Mikuláš, Herich Róbert
Department of Pathological Anatomy and Pathological Physiology, University of Veterinary Medicine and Pharmacy, Komenského 73, 040 01 Košice, Slovakia.
School of Biological Sciences and Engineering, Yachay-Tech University Hacienda San José, Imbabura, Urcuquí 100650, Ecuador.
Vet Sci. 2020 Aug 29;7(3):121. doi: 10.3390/vetsci7030121.
Due to the interest in using probiotic bacteria in poultry production, this research was focused on evaluating the effects of Biocenol CCM 7514 administration on body weight gain and cytokine gene expression in chickens challenged with . One-hundred and eight 1-day old COBB 500 broiler chickens were equally assigned to four experimental groups at random. In the control group (C) chicks were left untreated, whereas in groups LB and LBCj a suspension of was administered. A suspension of was subsequently applied to groups Cj and LBCj. Body weight was registered, and the individuals were later slaughtered; cecum samples were collected at 12, 36 and 48 h post-infection (hpi). The entire experiment lasted seven days. Reverse transcription quantitative PCR (RT-qPCR) was used to determine expression levels of IL-1β, IL-15, IL-17, and IL-18 at each time point. Pathogen-infected individuals were observed to weigh significantly less than those fed with the probiotic. Significant differences were also found in transcript abundance; expression of IL-15 was downregulated by the probiotic and upregulated by . The effects of bacterial treatments were time-dependent, as the expression profiles differed at later stages. The present outcomes demonstrate that both reduces the impact of infection on chicken body weight and regulates positively pro-inflammatory cytokine expression, which ultimately increase bird well-being and improves production.
由于在家禽生产中使用益生菌的兴趣，本研究重点评估了施用Biocenol CCM 7514对受[病原体名称未给出]攻击的鸡的体重增加和细胞因子基因表达的影响。108只1日龄的COBB 500肉鸡被随机均分为四个实验组。对照组（C）的雏鸡不进行处理，而在LB组和LBCj组施用[细菌名称未给出]的悬浮液。随后将[另一种细菌名称未给出]的悬浮液应用于Cj组和LBCj组。记录体重，随后宰杀个体；在感染后12、36和48小时（hpi）收集盲肠样本。整个实验持续7天。使用逆转录定量PCR（RT-qPCR）来确定每个时间点IL-1β、IL-15、IL-17和IL-18的表达水平。观察到受病原体感染的个体体重明显低于喂食益生菌的个体。在转录本丰度上也发现了显著差异；益生菌使IL-15的表达下调，而[病原体名称未给出]使其上调。细菌处理的效果是时间依赖性的，因为后期的表达谱有所不同。目前的结果表明，[Biocenol CCM 7514]既能降低[病原体名称未给出]感染对鸡体重的影响，又能正向调节促炎细胞因子的表达，最终提高鸡的健康水平并改善生产性能。
