Lee Hyun-Gwan, Kim Yoo-Bhin, Lee Sang-Hyeok, Moon Jun-Ok, Chae Jong-Pyo, Kim Yu-Jin, Lee Kyung-Woo
Department of Animal Science and Technology, Konkuk University, Seoul 05029, Korea.
CJ Cheiljedang Co., Ltd., Suwon 16495, Korea.
Vet Sci. 2022 Mar 7;9(3):119. doi: 10.3390/vetsci9030119.
The objectives of this study were to recover bacteriophages (BPs) from the intestinal digesta of BP-fed broilers and to evaluate the antibacterial effects of encapsulated or powdered BPs in broiler chickens challenged with . Day-old broiler chicks ( = 320/experiment) were randomly assigned to 32 pens ( = 10 broilers/pen) and allocated to one of four dietary groups: (1) unchallenged group (NEG); (2) -challenged group (POS); (3) POS group fed a diet supplemented with powdered BPs; and (4) POS group fed a diet supplemented with encapsulated BPs. On days 21, 22, and 23 post-hatch, all chickens except NEG were orally inoculated twice a day with 2 mL (1.0 × 10 cfu/mL). Varying BP levels were detected in gut digesta at all ages and were numerically or significantly higher in the encapsulated BP group than in the powdered BP group. Dietary powder or encapsulated BPs reversed the -mediated increase in crypt depth. In addition, villus height to crypt depth ratio was elevated in the NEG and BP-treated/challenged groups compared with that in the POS group. counts in the cecum were significantly lower in the BP-fed chickens than in the POS group. The encapsulated BP-supplemented diet-fed chickens had the highest serum IgA levels. Collectively, our results suggest that dietary BP remains viable in intestinal digesta upon ingestion and can inhibit cecal counts.
本研究的目的是从饲喂噬菌体的肉鸡肠道消化物中回收噬菌体（BPs），并评估包封或粉状BPs对受挑战的肉鸡的抗菌效果。1日龄的肉鸡雏鸡（每组320只）被随机分配到32个围栏（每围栏10只肉鸡），并分为四个日粮组之一：（1）未受挑战组（NEG）；（2）受挑战组（POS）；（3）饲喂补充粉状BPs日粮的POS组；（4）饲喂补充包封BPs日粮的POS组。在孵化后第21、22和23天，除NEG组外的所有鸡每天口服接种两次2 mL（1.0×10 cfu/mL）。在所有年龄段的肠道消化物中均检测到不同水平的BPs，包封BP组的BPs数量在数值上或显著高于粉状BP组。日粮中的粉状或包封BPs逆转了由介导的隐窝深度增加。此外，与POS组相比，NEG组以及BP处理/受挑战组的绒毛高度与隐窝深度之比升高。饲喂BP的鸡盲肠中的计数显著低于POS组。饲喂补充包封BP日粮的鸡血清IgA水平最高。总体而言，我们的结果表明，日粮中的BP在摄入后在肠道消化物中仍保持活性，并可抑制盲肠计数。
