基于聚合物磁性纳米颗粒和长春新碱药物的智能纳米制剂：肿瘤凋亡基因表达的新型疗法。

Smart Nanoformulation Based on Polymeric Magnetic Nanoparticles and Vincristine Drug: A Novel Therapy for Apoptotic Gene Expression in Tumors.

作者信息

Al-Musawi Sharafaldin, Ibraheem Sumayah, Abdul Mahdi Salih, Albukhaty Salim, Haider Adawiya J, Kadhim Afraa Ali, Kadhim Kadhim Ali, Kadhim Haitham Ali, Al-Karagoly Hassan

机构信息

Faculty of Biotechnology, Al-Qasim Green University, Babylon 51013, Iraq.

Al_kindy College of Medicine, University of Baghdad, Baghdad 10071, Iraq.

出版信息

Life (Basel). 2021 Jan 19;11(1):71. doi: 10.3390/life11010071.

BACKGROUND

Advanced nanobiotechnology provides safe and efficient drug delivery systems to deliver chemotherapy that targets cancer cells efficiently.

METHODS

A polymeric-magnetic nanocarrier was composed of a dextran (DEX) shell, a superparamagnetic iron oxide (SPION) core and was conjugated with folate (FA) to carry the anticancer drug vincristine (VNC) in Tera-1 testicular tumor cells. The molecular mechanisms by which apoptosis was induced were analyzed using flow cytometry and qPCR, which exhibited anticancer activity of nanoparticles (NPs).

RESULTS

This nanocarrier revealed a controlled release of VNC in citrate and phosphate buffer solutions that were maintained at pH 5.5 and pH 7.4, respectively. The Inhibitory concentration (IC50) values were greater than 5 mg/mL and displayed ten times higher cytotoxicity than the comparable free drug concentration. The Caspase-9 and P53 expressions were increased, whereas P21 and AKt1 decreased noticeably in the treated cells. The results point to the possible activation of apoptosis following treatment with NPs loaded with vincristine.

背景

先进的纳米生物技术提供了安全高效的药物递送系统，以有效地递送靶向癌细胞的化疗药物。

方法

一种聚合物磁性纳米载体由葡聚糖（DEX）外壳、超顺磁性氧化铁（SPION）核心组成，并与叶酸（FA）偶联，用于在Tera-1睾丸肿瘤细胞中运载抗癌药物长春新碱（VNC）。使用流式细胞术和qPCR分析诱导凋亡的分子机制，结果显示纳米颗粒（NPs）具有抗癌活性。

结果

该纳米载体在分别保持于pH 5.5和pH 7.4的柠檬酸盐和磷酸盐缓冲溶液中显示出VNC的控释。抑制浓度（IC50）值大于5 mg/mL，且显示出比相当的游离药物浓度高十倍的细胞毒性。在处理的细胞中，半胱天冬酶-9和P53表达增加，而P21和AKt1明显下降。结果表明用负载长春新碱的NPs处理后可能激活凋亡。