Gambino Delia, Vicari Domenico, Vitale Maria, Schirò Giorgia, Mira Francesco, Giglia Maria La, Riccardi Alessandra, Gentile Antonino, Giardina Susanna, Carrozzo Anna, Cumbo Valentina, Lastra Antonio, Gargano Valeria
Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Via Gino Marinuzzi n. 3, 90129 Palermo, Italy.
Department of Veterinary Science, University of Pisa, Viale delle Piagge n. 2, 56124 Pisa, Italy.
Microorganisms. 2021 Jan 19;9(1):203. doi: 10.3390/microorganisms9010203.
Wild environments and wildlife can be reservoirs of pathogens and antibiotic resistance. Various studies have reported the presence of zoonotic bacteria, resistant strains, and genetic elements that determine antibiotic resistance in wild animals, especially near urban centers or agricultural and zootechnical activities. The purpose of this study was the analysis, by cultural and molecular methods, of bacteria isolated from wild animals in Sicily, Italy, regarding their susceptibility profile to antibiotics and the presence of antibiotic resistance genes. Bacteriological analyses were conducted on 368 wild animals, leading to the isolation of 222 bacterial strains identified by biochemical tests and 16S rRNA sequencing. The most isolated species was , followed by and . Antibiograms and the determination of resistance genes showed a reduced spread of bacteria carrying antibiotic resistance among wild animals in Sicily. However, since several wild animals are becoming increasingly close to residential areas, it is important to monitor their health status and to perform microbiological analyses following a One Health approach.
野生环境和野生动物可能是病原体和抗生素耐药性的储存库。各种研究报告称，在野生动物中存在人畜共患病细菌、耐药菌株以及决定抗生素耐药性的遗传元件，尤其是在城市中心附近或农业及畜牧活动区域。本研究的目的是通过培养和分子方法，分析从意大利西西里岛的野生动物中分离出的细菌，了解它们对抗生素的敏感性概况以及抗生素耐药基因的存在情况。对368只野生动物进行了细菌学分析，通过生化试验和16S rRNA测序鉴定出222株细菌菌株。分离出最多的物种是 ，其次是 和 。药敏试验和耐药基因测定表明，在西西里岛的野生动物中，携带抗生素耐药性的细菌传播有所减少。然而，由于越来越多的野生动物靠近居民区，监测它们的健康状况并采用“同一健康”方法进行微生物学分析非常重要。
